merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB13405,Anti-MMP-2 Proform Antibody, NT, clone CA-4001

  • merck millipore,默克密理博,MAB13405,Anti-MMP-2 Proform Antibody, NT, clone CA-4001

    产品名称：Anti-MMP-2 Proform Antibody, NT, clone CA-4001
    产品型号：MAB13405
    Anti-MMP-2 Proform Antibody, N-terminus, clone CA-4001 detects level of MMP-2 Proform & has been published & validated for use in WB, IH(P).

    merck millipore,默克密理博,MAB13405,Anti-MMP-2 Proform Antibody, NT, clone CA-4001

  • 产品介绍
  • merck millipore,默克密理博,MAB13405,Anti-MMP-2 Proform Antibody, NT, clone CA-4001

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB13405
    Replaces 04-1048
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MMP-2 Proform Antibody, NT, clone CA-4001
    Alternate Names
    • 72 kDa Type IV Collagenase
    • Gelatinase A
    产品信息
    格式 Purified
    控制
    • POSITIVE CONTROL: HFL-1 cells. Conditioned, serum-free medium from (TPA-treated) human fetal lung cells. Placenta.
    演示 Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide
    应用
    应用 Anti-MMP-2 Proform Antibody, N-terminus, clone CA-4001 detects level of MMP-2 Proform & has been published & validated for use in WB, IH(P).
    主要应用
    • Western Blotting
    应用说明 Western Blot (0.5-1.0 μg/mL for 2 hours at room temperature, non-reduced conditions. However, chicken samples react best when fully reduced. Antibody dilution buffers should be simple for best results, PBS or TBS with 0.05% tween only. Immunogen used is highly conserved between species, thus immunoabsorption to pro-MMP2 in milk may impair reactivity.

    Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections: 2-10 μg/mL for 30 minutes at room temperature. {Seftor, et al 2001} and Gottsch ML et al 2002}. Antigen retrieval is optional in most cases. Antibody dilution solutions should not contain serum because of possible immunoabsorption of the antibody to proMMP2 present in the solution.

    Blocking: Inhibition of MMP-2 Activity (2-4 μg/mL).



    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 N-terminal peptide APSPIIKFPGDVAPKTDK of procollagenase IV, coupled to KLH.{Marqulies, et al 1992}
    表位 N-terminus
    克隆 CA-4001
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 It recognizes a protein of 72 kDa which is identified as pro (latent) form (active form is 62 kDa) of matrix metalloproteinase-2 and any fragment with N-terminal (proform) domain still attached. Antibody shows no cross-reaction with pro and active forms of MMP-9. MMP-2 is synthesized as 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). Cellular Localization: Cytoplasmic.
    同种型 IgG1
    品种反应性 RatHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_004530.2
    Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome.
    基因符号
    • EC 3.4.24.24
    • MMP2
    • TBE-1
    • CLG4A
    • CLG4
    • MMP-II
    • MONA
    • MMP-2
    UniProt编号
    • P08253
    UniProt汇总 FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
    COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
    SIZE: 660 amino acids; 73882 Da
    SUBUNIT: Ligand for integrin alpha-V/beta-3.
    TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
    DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
    DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
    SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB13405,Anti-MMP-2 Proform Antibody, NT, clone CA-4001

上一件merck millipore产品：merck millipore,默克密理博,178276,Apicidin, Fusarium sp. - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,SCR066,Quantitative Alkaline Phosphatase ES Characterization Kit


相关产品

merck millipore,默克密理博,ABE361,Anti-acetyl-Histone H4 (Lys91) Antibody merck millipore,默克密理博,ABE361,Anti-acetyl-Histone H4 (Lys91) Antibody

merck millipore,默克密理博,APT429,CaspaTag Caspase 9 In Situ Assay Kit, Fluorescein merck millipore,默克密理博,APT429,CaspaTag Caspase 9 In Situ Assay Kit, Fluorescein

merck millipore,默克密理博,MAB834-2,Anti-Parainfluenza 1 Antibody, clone 253/9D merck millipore,默克密理博,MAB834-2,Anti-Parainfluenza 1 Antibody, clone 253/9D

merck millipore,默克密理博,ABC829,Anti-SCRN1 Antibody merck millipore,默克密理博,ABC829,Anti-SCRN1 Antibody

merck millipore,默克密理博,178276,Apicidin, Fusarium sp. - Calbiochem merck millipore,默克密理博,178276,Apicidin, Fusarium sp. - Calbiochem

merck millipore,默克密理博,SCR066,Quantitative Alkaline Phosphatase ES Characterization Kit merck millipore,默克密理博,SCR066,Quantitative Alkaline Phosphatase ES Characterization Kit

merck millipore,默克密理博,AG19P-K,Human Spleen Ferritin, Purified (1mg) merck millipore,默克密理博,AG19P-K,Human Spleen Ferritin, Purified (1mg)

merck millipore,默克密理博,MABF510,Anti-CD117 (c-Kit) Antibody (mouse), APC, clone ACK2 merck millipore,默克密理博,MABF510,Anti-CD117 (c-Kit) Antibody (mouse), APC, clone ACK2

merck millipore,默克密理博,AB4305,Anti-Pramel-5 Antibody merck millipore,默克密理博,AB4305,Anti-Pramel-5 Antibody

merck millipore,默克密理博,AB6031,Anti-Integrin alpha-11 Antibody merck millipore,默克密理博,AB6031,Anti-Integrin alpha-11 Antibody


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部