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merck millipore,默克密理博,MAB1207,Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1
产品名称：Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1
产品型号：MAB1207
This Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1 is validated for use in FC, IC for the detection of Platelet IIb-IIIa.
merck millipore,默克密理博,MAB1207,Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1207 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1 Alternate Names - CD41
产品信息 格式 Purified 演示 The antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by electrophoresis and flow cytometry. 应用 应用 This Anti-Platelet IIb-IIIa Antibody, clone 290AS-10. a.k.a. HuPl-m1 is validated for use in FC, IC for the detection of Platelet IIb-IIIa. 主要应用 - Immunocytochemistry
- Flow Cytometry
应用说明 This antibody is useful in biochemical studies of the Glycoprotein IIb-IIIa complex and the enumeration of platelets. The antibody specifically inhibits ADP and collagen induced aggregation and release of ATP, and ADP induced platelet fibrinogen binding1. The antibody may also be used for immunoperoxidase staining of formalin fixed cell suspensions and frozen tissue sections.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1x10E6 platelets in 100 μl of buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.生物信息 免疫原品种 Human Platelet membranes. 克隆 290AS-10. a.k.a. HuPl-m1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The specificity of this antibody is equivalent to that of CD41 monoclonal antibodies, and it detects the Glycoprotein IIb-IIIa complex on the surface of platelets and megakaryocytes. It is not reactive with T and B cells or with other haemopoietic cells. The antibody precipitates a complex of molecular weights 122kD and 112kD when reduced and 87kD and 140kD when not reduced1. The IIb-IIIa complex is a member of the integrin superfamily of adhesive receptors and mediates platelet-platelet aggregation. This complex recognizes the Arg-Gly-Asp (RGD) tripeptide sequence in adhesive glycoproteins such as fibronectin, fibrinogen and von Willebrand Factor thereby facilitating linkage between adjacent platelets. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000419.3
Entrez基因汇总 ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. 基因符号 - GTA
- ITGA2B
- CD41B
- HPA3
- CD41
- GPIIb
- GP2B
- ITGAB
UniProt编号 - P08514
UniProt汇总 FUNCTION: SwissProt: P08514 # Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
SIZE: 1039 amino acids; 113391 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
DISEASE: SwissProt: P08514 # Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. This autosomal recessive disorder is the most common inherited disease of platelets. GT is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
SIMILARITY: SwissProt: P08514 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.包装信息 数量 100 µg
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