merck millipore,默克密理博,69048,T7•Tag Antibody HRP Conjugate

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merck millipore,默克密理博,69048,T7•Tag Antibody HRP Conjugate
产品名称：T7•Tag Antibody HRP Conjugate
产品型号：69048

merck millipore,默克密理博,69048,T7•Tag Antibody HRP Conjugate

产品介绍

merck millipore,默克密理博,69048,T7•Tag Antibody HRP Conjugate

描述

概述

The T7•Tag Antibody HRP Conjugate is useful as a single detection reagent and eliminates the need for secondary antibodies or streptavidin conjugates.The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

The T7•Tag^® Antibody is a mouse monoclonal antibody (IgG_2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase.

Specificity	T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/Isotype	Mouse monoclonal IgG_2b
Cross-reactivity	Negligible with bacterial, insect or mammalian cell lysates
Sensitivity	1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (developed with chemiluminescent substrates)
Form	Stabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution	1:5,000–1:10,000