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品种反应性 主要应用 宿主 格式 抗体类型 H, M ICC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MAB10404 描述 Anti-Ghrelin Antibody, clone 1ML-1D7 Alternate Names - Growth hormone secretagogue
- ghrelin/obestatin prepropeptide1
- ghrelin/obestatin2
- ghrelin, growth hormone secretagogue receptor ligand1
- obestatin1
- ghrelin/obestatin preprohormone
- Motilin-related peptide2
- ghrelin1
- M46 protein3
背景信息 Ghrelin is a 28 amino acids peptide with an essential n-octonayl modification at Serine 3. This peptide hormone is known as an endogenous ligand for the growth hormone secretagogue receptor and shares sequence similarity with the peptide hormone Motilin. Ghrelin displays pleotropic actions including growth hormone secretion, feeding behaviour and gastrointestinal motility. It is predominantly produced by enteroendocrine cells from the gastrointestinal tract, but has been shown to play a significant role in neurotrophy, particularly in the hippocampus, and appears to be essential for cognitive adaptation to changing environments and the process of learning. 产品信息 格式 Ascites 控制 - Mouse stomach tissue lysate
演示 Unpurified mouse monoclonal ascites with 0.05% sodium azide. 应用 应用 Detect Ghrelin using this Anti-Ghrelin Antibody, clone 1ML-1D7 validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ghrelin in A431 cells. 生物信息 免疫原品种 Linear Peptide corresponding to the N-terminus of human Ghrelin. 表位 N-terminus 克隆 clone 1ML-1D7 宿主 Mouse 特异性 This antibody recognizes Ghrelin at the N-terminus. 同种型 IgG1κ 品种反应性 HumanMouse Species Reactivity Note Demonstrated to react with human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_057446.1
Entrez基因汇总 This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq]. 基因符号 - GHRL
- MTLRP1
纯化方法 Unpurified UniProt编号 - Q9UBU3
UniProt汇总 FUNCTION: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility By similarity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity by similarity.
SEQUENCE SIMILARITIES: Belongs to the motilin family.
MASS SPECTROMETRY: Molecular mass is 3398.9±0.3 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10, O-decanoylated form.
Molecular mass is 3397.2±0.5 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10:1, O-decenoylated form.
Molecular mass is 3371.3±0.1 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C8, O-octanoylated form.
Molecular mass is 3243.6±0.4 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C10, O-decanoylated form.
Molecular mass is 3214.6±0.6 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C8, O-octanoylated form.产品使用声明 质量保证 Evaluated by Western Blot in mouse stomach tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Ghrelin in 10 µg of mouse stomach tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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