merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

  • merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

    产品名称：Anti-Tumor Necrosis Factor-α Antibody, clone 2C8
    产品型号：MAB1021
    Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.

    merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

  • 产品介绍
  • merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, ELISAMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB1021
    Replaces 04-1114
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Tumor Necrosis Factor-α Antibody, clone 2C8
    Alternate Names
    • TNF-alpha
    产品信息
    格式 Purified
    演示 Liquid in PBS pH 7.4 containing 0.1% sodium azide.
    应用
    应用 Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
    主要应用
    • Immunohistochemistry
    • ELISA
    应用说明 Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
    Detection of TNF-α in two-site immunoassay.

    Immunohistochemistry detected TNF-α.

    TNF-α Neutralization Assay

    1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.

    2. Incubate overnight at 37°C in humidified 5% CO2 incubator.

    3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL

    4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.

    5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.

    6. Incubate 24 hours at 37°C, 5% CO2.

    7. Score wells for cytotoxicity.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Human recombinant tumor necrosis factor α.
    克隆 2C8
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Human tumor necrosis factor-a (TNF-a)
    同种型 IgG1
    品种反应性 Human
    Species Reactivity Note Human. No cross-reactivity observed with rat neuronal tissue.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000594.2
    Entrez基因汇总 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
    基因符号
    • DIF
    • TNF
    • TNFSF2
    • Cachectin
    • TNF-alpha
    • OTTHUMP00000037669
    • TNFA
    • cachectin
    • TNF-a
    纯化方法 Protein A purfied
    UniProt编号
    • P01375
    UniProt汇总 FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    SIZE: 233 amino acids; 25644 Da
    SUBUNIT: Homotrimer.
    SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
    PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
    SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C in undiluted aliquots for up to one year.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

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