- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS100M,ChemiSCREEN™ Human δ Opioid Receptor Membrane Preparation
产品名称：ChemiSCREEN™ Human δ Opioid Receptor Membrane Preparation
产品型号：HTS100M
Human Delta / OP1 / DOP / DOR GPCR membrane preparation for Radioligand binding Assays & GTPγS.
merck millipore,默克密理博,HTS100M,ChemiSCREEN™ Human δ Opioid Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS Human Chem-1 Delta / OP1 / DOP / DOR 描述 产品目录编号 HTS100M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human δ Opioid Receptor Membrane Preparation 概述 Full-length human OPRD1 cDNA encoding δ opioid receptor Alternate Names - DOR, OP1
背景信息 Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, and μ (also known as OP1, OP2 and OP3, resepctively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the µ opioid receptor, agonits of spinal δ opioid receptors have antinociceptive activity that is independent of µ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Chemicon's δ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at δ. The membrane preparations exhibit a Kd of 1.12 nM for [3H]-DADLE. With 0.9 nM [3H]-DADLE, 10 µg/well δ Membrane Prep typically yields greater than 4-fold signal-to-background ratio. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 3H-labeled DADLE at 0.9 nM
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.应用 应用 Human Delta / OP1 / DOP / DOR GPCR membrane preparation for Radioligand binding Assays & GTPγS. 应用说明 Radioligand binding assay and GTPγS 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_000911.3
基因符号 - DOR-1
- OPRD1
- OPRD
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 Delta / OP1 / DOP / DOR 靶子系列 Opioid UniProt编号 - P41143
UniProt汇总 FUNCTION: SwissProt: P41143 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
SIZE: 372 amino acids; 40413 Da
SUBUNIT: Interacts with GPRASP1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P41143 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris-HCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Tris-HCl, filtered and stored at 4°C
Radioligand: [3H] DADLE (Perkin Elmer#: NET-648)
Wash Buffer: 50 mM Tris-HCl, filtered and stored at 4°C.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with δ membrane prep and unlabeled DPDPE.
10 µg/well Signal:Background 7.32 Specific Binding (cpm) 955
SPECIFICATIONS: 1 unit = 10 µg
Bmax for [3H] DADLE binding: 11.6 pmol/mg protein
Kd for [3H] DADLE binding: ~1.12 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,AB15138,Anti-Nogo Receptor Antibody
下一件merck millipore产品：merck millipore,默克密理博,437620,Lipopolysaccharide, E. coli J5 - Calbiochem
相关产品
merck millipore,默克密理博,GF003,Fibroblast Growth Factor basic Protein, Human recombinant
merck millipore,默克密理博,MABT171,Anti-MMP-9 (Active) Antibody, clone 4A3
merck millipore,默克密理博,181315,ARE Activator, BTZO-1 - Calbiochem
merck millipore,默克密理博,14-805M,Met (Y1248C) Protein, active, 250 µg
merck millipore,默克密理博,AB15138,Anti-Nogo Receptor Antibody
merck millipore,默克密理博,437620,Lipopolysaccharide, E. coli J5 - Calbiochem
merck millipore,默克密理博,MAB1021,Anti-Tumor Necrosis Factor-α Antibody, clone 2C8
merck millipore,默克密理博,05-479,Anti-Cytochrome C Antibody, clone C-7
merck millipore,默克密理博,SCM110,Human ES/iPS Neural Induction Medium
merck millipore,默克密理博,20-154,Low Salt Immune Complex Wash Buffer