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描述 产品目录编号 LSKGEL050 商名 - Montage
描述 Montage 凝胶回收试剂盒 概述 Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.- Minimal hands-on time
- 10-minute spin
- Fully-functional DNA
PerformanceTypical DNA Recoveries from Agarose Gels
DNA Size (bp) Percent DNA Recoveries
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.Alternate Names - Ultrafree-DA
产品信息 部件 - 包含 50 个 Ultrafree-DA 装置和 500 mL 的 50x 浓缩
改良 TAE 缓冲液
色码 蓝色 应用 应用 DNA Gel Extraction 材料信息 装置材料 - Polypropylene
包装信息 数量 50
上一件merck millipore产品：merck millipore,默克密理博,07-1817,Anti-phospho-SRPK2 (Ser494) Antibody
下一件merck millipore产品：merck millipore,默克密理博,04-292,Anti-phosho-erbB2/Her2 (Ser1113) Antibody, clone 9E10
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