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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB16926 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-DFF40 Antibody Alternate Names - CPAN
- DNA Fragmentation Factor, 40 kDa Subunit
- Caspase-activated DNase
- CAD
- CAD
产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 Detect DFF40 using this Anti-DFF40 Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:500-1:1,000
K562 or Jurkat whole cell lysate can be used as a positive control and a 40 kDa band should be detected.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A peptide corresponding to amino acids 147-164 of mouse CAD (Enari et al. 1998). The sequence differs from human DFF40 by two amino acids (Halenbeck et al. 1998; Liu et al. 1998). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (Enari et al. 1998; Sakahira et al. 1998). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN and DFF40 and human CAD, respectively (Halenbeck et al. 1998; Liu et al. 1998; Mukae et al. 1998). DFF45/ICAD is the inhibitory protein of DFF40/CAD (Enari et al. 1998; Sakahira et al. 1998; Liu et al. 1997) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually cuases the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004402.2
- NM_001004285.1
- NM_001004286.1
Entrez基因汇总 Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. 基因符号 - EC 3.-.-.-
- DFFB
- CAD
- DFF40
- DFF-40
- CPAN
- DFF2
UniProt编号 - O76075
UniProt汇总 FUNCTION: SwissProt: O76075 # Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
SIZE: 338 amino acids; 39110 Da
SUBUNIT: Heterodimer of DFFA and DFFB.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
SIMILARITY: SwissProt: O76075 ## Contains 1 CIDE-N domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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