merck millipore,默克密理博,HTS206RTA,Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,HTS206RTA,Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells
产品名称：Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells
产品型号：HTS206RTA
Human MRGPRD / MrgD GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS206RTA,Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS206RTA,Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	MRGPRD / MrgD

描述
产品目录编号	HTS206RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The Mas-related genes (Mrgs), also known as sensory neuron-specific G-protein coupled receptors (SNSRs), are selectively expressed in subpopulations of sensory neurons involved in the perceptor of pain (Dong et al., 2001). The Mrg family in rodents consists of 50 receptors in the MrgA-MrgH subfamilies, but humans contain fewer Mrg members. MRGPRD (also known as MrgD and TGR7) is found across species from rodents to humans, and its expression is restricted to nonpeptidergic, small-diameter, IB4+ dorsal root ganglion (DRG) neurons. MRGPRD binds β-alanine, but not L-alanine, which results in increased intracellular calcium and the suppression of forskolin-induced cAMP production, thus suggesting coupling to Gq and Gi proteins (Shinohara et al., 2004). β-alanine-induced activation of MRGPRD inhibits KCNQ2/3-mediated M-currents in cells overexpressing MRGPRD and KCNQ2/3 and also in cultured DRG neurons, which indicates that MRGPRD enhances nociceptive signaling (Crozier et al., 2007). Millipore’s cloned human MRGPRD-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MRGPRD expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MRGPRD.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human MRGPRD / MrgD GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	AY427820 NM_198923
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	MRGPRD / MrgD
靶子系列	Mas-related gene
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

包装信息
数量	1 kit

merck millipore,默克密理博,HTS206RTA,Ready-to-Assay™ MRGPRD Mas-Related Gene Receptor Frozen Cells

上一件merck millipore产品：merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4
下一件merck millipore产品：merck millipore,默克密理博,AB2263,Anti-MEF2D Antibody

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech