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品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, R, M, Po, Eq, Rhesus Macaque WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2263 描述 Anti-MEF2D Antibody Alternate Names - myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusion protein
- myocyte enhancer factor 2D
- MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D)
- MEF2D/DAZAP1 fusion
- myocyte-specific enhancer factor 2D
背景信息 Myocyte-specific enhancer factor 2D is a transcriptional activator belonging to the MEF2 family. MEF2D has two domains of interest; a MADS-box domain, and a Mef2-type DNA-binding domain specifically binding to 5′-YTA[AT]4TAR-3′, the MEF2 element, which is observed in several gene types such as growth factor, muscle-specific, and stress-induced genes. MEF2D regulates various cellular processes including those within cardiac and skeletal muscle development, neuronal survival and differentiation, as well as cell growth, apoptosis and survival. MEF2D, along with ERK5, plays a vital role in neuronal survival by regulating the anti-apoptotic molecule, bcl-w. There are 6 known isoforms of MEF2D resulting from alternative splicing. 产品信息 格式 Affinity Purified 控制 - L6 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-MEF2D Antibody is an antibody against MEF2D for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected MEF2D in normal human breast skin and inflammatory skin tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human MEF2D. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 CanineHumanRatMousePigHorseRhesus Macaque Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with porcine, equine, rhesus monkey, and canine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005911
基因符号 - MEF2D
纯化方法 Affinity purified UniProt编号 - Q14814
UniProt汇总 FUNCTION: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).
SUBUNIT STRUCTURE: Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with HDAC4 (in undifferentiating cells); the interaction translocates MEF2D to nuclear dots. Forms a heterodimer with MEF2A.
SUBCELLULAR LOCATION: Nucleus. Note: Translocated by HDAC4 to nuclear dots.
DEVELOPMENTAL STAGE: Present in myotubes and also in undifferentiated myoblasts.
DOMAIN: The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
PTM: Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation (By similarity).
SEQUENCE SIMILARITIES: Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain.产品使用声明 质量保证 Evaluated by Western Blot in L6 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MEF2D on 10 µg of L6 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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