merck millipore,默克密理愽
  • merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4

  • merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4

    产品名称：Anti-SIRT1 Antibody, clone 10E4
    产品型号：04-1557
    Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtu More>>

    merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4

  • 产品介绍
  • merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HChIP, WB, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-1557
    描述 Anti-SIRT1 Antibody, clone 10E4
    Alternate Names
    • sirtuin type 1
    • SIR2-like protein 1
    • sir2-like 1
    • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
    • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
    • sirtuin 1
    背景信息 Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
    产品信息
    格式 Purified
    控制
    • HeLa cell lysate
    演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1.
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunoprecipitation: Reported by independent researcher using representative lot.
    Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
    Immunohistochemistry: Reported by independent researcher using representative lot.
    生物信息
    免疫原品种 His-tagged recombinant protein corresponding to human SIRT1.
    表位 Unknown
    克隆 10-E-4
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes SIRT1.
    品种反应性 MouseHuman
    Species Reactivity Note Demonstrated to react with human. Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_036370.2
    Entrez基因汇总 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
    基因符号
    • SIR2alpha
    • SIRT1
    • SIR2L1
    • hSIR2
    • hSIRT1
    纯化方法 Protein G purfied
    UniProt编号
    • Q96EB6
    UniProt汇总 FUNCTION: NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2- mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.

    COFACTOR: Binds 1 zinc ion per subunit (By similarity).

    SIZE: 747 amino acids; 81681 Da

    SUBUNIT: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity). Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML.

    SUBCELLULAR LOCATION: Nucleus. Note=Recruited to the nuclear bodies via its interaction with PML.TISSUE SPECIFICITY: Widely expressed.

    SIMILARITY: Belongs to the sirtuin family. & Contains 1 deacetylase sirtuin-type domain.

    MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa cell lysate.

    Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,04-1557,Anti-SIRT1 Antibody, clone 10E4

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