merck millipore,默克密理博,HTS189M,ChemiSCREEN™ Membrane Preparation Recombinant Human GPR103 / QRFP Neuropeptide Receptor

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay and GTPγS binding	Human	Chem-1	GPR103 / QRFP

描述
产品目录编号	HTS189M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ Membrane Preparation Recombinant Human GPR103 / QRFP Neuropeptide Receptor
概述	Human GPR103
背景信息	GPR103 is a Gq-coupled receptor that is located in the brain and adrenal gland, and is activated by a 43-amino acid RFamide peptide, QRFP43, and a 26 amino acid fragment, QRFP26/26RFa/Peptide 518 (Jiang et al., 2003; Fukusumi et al., 2003). QRFP43 is the active form in rat brain, and intracerebral administration of QRFP43 induces feeding and metabolic rate (Takayasu et al., 2006; Moriya et al., 2006). Millipore's GPR103 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GPR103. The membrane preparations exhibit a Kd of 0.63nM for [¹²⁵I]-QRFP43. With 10 μg/well GPR103 Membrane Prep and 0.35 nM [¹²⁵I]- QRFP43, a greater than 6-fold signal-to-background ratio was obtained.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human GPR103 / QRFP GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay and GTPγS binding

生物信息
品种	Human
Entrez基因编号	NM_198179
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	GPR103 / QRFP
靶子系列	Peptide P518
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl₂, 1 mM CaCl₂, 0.2% BSA, filtered and stored at 4°C Radioligand: [¹²⁵I]QRFP43 (Perkin Elmer # NEX408) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 6-fold signal:background with ¹²⁵I-labeled QRFP43 at 0.35 nM.

产品使用声明

质量保证

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of GPR103 membrane prep.

	10 µg/well
Signal:Background	10
Specific Binding (cpm)	4217

SPECIFICATIONS: 1 unit = 10 µg
Bmax: 0.81 pmol/mg
Kd: 0.63 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

包装信息
数量	200 units

merck millipore,默克密理博,HTS189M,ChemiSCREEN™ Membrane Preparation Recombinant Human GPR103 / QRFP Neuropeptide Receptor

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