merck millipore,默克密理博,17-320,Histone Deacetylase Assay Kit (HDAC)

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,17-320,Histone Deacetylase Assay Kit (HDAC)
产品名称：Histone Deacetylase Assay Kit (HDAC)
产品型号：17-320
This kit contains nonradioactive components for radiolabeling & purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity.

merck millipore,默克密理博,17-320,Histone Deacetylase Assay Kit (HDAC)

产品介绍

merck millipore,默克密理博,17-320,Histone Deacetylase Assay Kit (HDAC)

重要规格表

主要应用
EA

描述
产品目录编号	17-320
品牌系列	Upstate
商名	Upstate
描述	Histone Deacetylase Assay Kit (HDAC)
概述	This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.

产品信息
部件	PCAF active HeLa Nuclear Extract (Cat.# 12-309) Histone H4 Peptide, biotin conjugate, residues 2-24 (Cat.# 12-372) HAT Assay Buffer, 5X (Cat.# 20-148) Sodium Butyrate (Cat.# 19-137) 5X HDAC Assay Buffer Streptavidin Agarose

应用
应用	This kit contains nonradioactive components for radiolabeling & purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity.
主要应用	Enzyme Assay

生物信息
Entrez基因编号	NM_004964.2
Entrez基因汇总	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
基因符号	RPD3 HDAC1 RPD3L1 GON-10 DKFZp686H12203 HD1
UniProt编号	Q96DB2 Q13547 Q92769 O15379 P56524 Q9UQL6 Q9UBN7 Q8WUI4 Q9BY41 Q9UKV0 Q969S8
UniProt汇总	FUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. SIZE: 1122 amino acids; 121992 Da SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity). PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. & Ubiquitinated. Polyubiquitination however does not lead to its degradation. SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.

产品使用声明
质量保证	Routinely evaluated with two experiments, The dependence of HDAC activity on HeLa Nuclear Extract and the inhibition of HDAC Activity by Sodium Butyrate. In the first experiment, HeLa nuclear extract was incubated at room temperature with [³H]-acetyl-Histone H4 peptide. Released [³H]-acetate was measured using a scintillation counter. In the second experiment, [³H]-acetyl-Histone H4 peptide was incubated with HeLa nuclear extract in the presence or absence of Sodium Butyrate. Sodium Butyrate inhibited release of [³H]-acetate by ~92%.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated with two experiments, The dependence of HDAC activity on HeLa Nuclear Extract and the inhibition of HDAC Activity by Sodium Butyrate.

In the first experiment, HeLa nuclear extract was incubated at room temperature with [³H]-acetyl-Histone H4 peptide. Released [³H]-acetate was measured using a scintillation counter.

In the second experiment, [³H]-acetyl-Histone H4 peptide was incubated with HeLa nuclear extract in the presence or absence of Sodium Butyrate. Sodium Butyrate inhibited release of [³H]-acetate by ~92%.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.