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merck millipore,默克密理博,HTS180M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW1 Neuropeptide B/W Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW1 Neuropeptide B/W Receptor
产品型号：HTS180M
Human NPBW1 / GPR7 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS180M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW1 Neuropeptide B/W Receptor
- 产品介绍
- merck millipore,默克密理博,HTS180M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW1 Neuropeptide B/W Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 NPBW1 / GPR7 描述 产品目录编号 HTS180M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW1 Neuropeptide B/W Receptor 概述 Full length human GPR7 cDNA encoding NPBW1 背景信息 Neuropeptide B (NPB) and neuropeptide W (NPW) are members of a recently identified neuropeptide family that are ligands for two highly similar receptors NPBW1 and NPBW2, both of which are coupled to Gi/o protein to inhibit intracellular cAMP production. Highest expression of NPBW1 mRNA and protein was identified in the amygdala and hypothalamic nuclei. Physiological studies demonstrate that intracerebroventricular infusion of NPBW1 ligands modulates feeding behavior, regulates the release of corticosterone, prolactin and growth hormone while also modulating pain pathway (Singh and Davenport, 2006). NPBW1 knock out male mice have shown mild adult-onset obesity and decreased locomotor activity. They become progressively hyperglycaemic and hyperinsulinaemic (Ishii et al., 2003). Millipore's NPBW1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NPBW1 receptor interactions with its ligand. The membrane preparations exhibit a Kd of 0.53nM for [125I]-Neuropeptide W23. With 5 ug/well NPBW1 Membrane Prep and 0.35nM [125I]-Neuropeptide W23, a greater than 10-fold signal-to-background ratio was obtained. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.应用 应用 Human NPBW1 / GPR7 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_005285.3
基因符号 - MGC129755
- NPBWR1
- GPR7
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 NPBW1 / GPR7 靶子系列 Neuropeptide B/W UniProt编号 - P48145
UniProt汇总 FUNCTION: SwissProt: P48145 # Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.
SIZE: 328 amino acids; 36117 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Found in cerebellum and frontal cortex. Detected at high levels in hipppocampus, amygdala and trachea; at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.
SIMILARITY: SwissProt: P48145 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C.
Radioligand: [125I]-neuropeptide W23 (Perkin Elmer#: NET-403)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled neuropeptide W23 at 0.35nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with 5 µg/well of NPBW1 Receptor membrane prep. 5µg/well Signal:Background 31.8 Specific Binding (cpm) 21932
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 8.74pmol/mg
Kd: 0.53 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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