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merck millipore,默克密理博,HTS170M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor
产品型号：HTS170M
Human sst1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS170M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS170M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 sst1 描述 产品目录编号 HTS170M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor 概述 Human sst1 背景信息 Somatostatin, a 14 or 28 amino acid peptide, inhibits hormone secretion from exocrine glands, and displays an antiproliferative activity on a number of cell types. A family of 5 Gi-coupled receptors mediates the effects of somatostatin. The classical activity of somatostatin of inhibiting growth hormone release from the pituitary appears to be mediated by sst1. In addition, sst1 functions as an inhibitory autoreceptor on somatostatin neurons in the hippocampus, hypothalamus, basal ganglia and retina (Thermos et al., 2006). The somatostatin receptors are targets for anticancer drugs and for treatment of hormonal dysfunction. Millipore's sst1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of sst1. The membrane preparations exhibit a Kd of 1.4 nM for [125I]- somatostatin-14. With 0.5 nM [125I]- somatostatin-14, 5 μg/well sst1 Membrane Prep yields greater than 12 fold signal-to-background ratio. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 12-fold signal:background with [125I]- somatostatin-14 at 0.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
应用 应用 Human sst1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_000868
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 sst1 靶子系列 Somatostatin 孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]- somatostatin-14 (PerkinElmer NEX389)
Wash Buffer: 50 mM HEPES, pH 7.4, 500 mM NaCl, 0.1% BSA, filtered and stored at 4°C.
产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of sst1 membrane prep.
5 µg/well Signal:Background 20.4 Specific Binding (cpm) 14,647
SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 8.4 pmol/mg
Kd: 1.4 nM
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
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