merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3565,Anti-PARP (214/215) cleavage site Antibody

  • merck millipore,默克密理博,AB3565,Anti-PARP (214/215) cleavage site Antibody

    产品名称：Anti-PARP (214/215) cleavage site Antibody
    产品型号：AB3565
    Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.

    merck millipore,默克密理博,AB3565,Anti-PARP (214/215) cleavage site Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB3565,Anti-PARP (214/215) cleavage site Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HICC, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3565
    Replaces 04-575; 04-576
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-PARP (214/215) cleavage site Antibody
    Alternate Names
    • ARTD1
    • Poly[ADP-ribose] synthase 1
    • ADPRT 1
    • NAD(+) ADP-ribosyltransferase 1
    • PARP-1
    • Poly [ADP-ribose] polymerase 1
    背景信息 Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
    产品信息
    格式 Affinity Purified
    控制
    • Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).
    演示 Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
    应用
    应用 Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.
    生物信息
    免疫原品种 The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.
    宿主 Rabbit
    特异性 Human and mouse (86% conserved) PARP. Hamster and rat (86% conserved) PARP have not been tested, but are expected to react.
    品种反应性 MouseHuman
    Species Reactivity Note Hamster and rat (86% homologous) PARP have not been tested, but are expected to react.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_001618.3
    Entrez基因汇总 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
    基因符号
    • EC 2.4.2.30
    • PARP1
    • poly(ADP-ribosyl)transferase
    • PARP-1
    • PARP
    • NAD
    • pADPRT-1
    • ADPRT1
    • Poly
    • ADPRT
    • PPOL
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P09874
    UniProt汇总 FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
    SIZE: 1014 amino acids; 113084 Da
    SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
    SUBCELLULAR LOCATION: Nucleus.
    PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
    SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers.
    MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
    产品使用声明
    质量保证 Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB3565,Anti-PARP (214/215) cleavage site Antibody

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