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merck millipore,默克密理博,HTS159M,ChemiSCREEN™ Membrane Preparation Recombinant Human β3 Adrenoceptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human β3 Adrenoceptor
产品型号：HTS159M
Human beta 3 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS159M,ChemiSCREEN™ Membrane Preparation Recombinant Human β3 Adrenoceptor
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 beta 3 描述 产品目录编号 HTS159M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human β3 Adrenoceptor 概述 Full-length human ADRB3 cDNA encoding the β3 adrenoceptor Alternate Names - beta3
背景信息 The beta adrenergic receptors mediate the effects of endogenous catecholamines, such as epinephrine, by coupling to Gs to stimulate cAMP . Whereas β1 and β2 are found predominantly in heart, the β3 receptor is found primarily in adipose tissue. Activation of adipose β3 results in lipolysis and thermogenesis. A polymorphism in the human gene for β3 is associated with weight gain in obese patients (Clement et al., 1995). In addition, mice lacking the β3-adrenoceptor display increased total body fat, particularly on a high fat diet (Revelli et al., 1997). These observations indicate that β3 is a possible target for obesity treatments. Millipore's β3 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β3 adrenoceptor interactions. The membrane preparations exhibit a Kd of 0.69 nM for [125I]-(-) Iodocyanopindolol (ICYP). With 10 µg/well β;3 Adrenoceptor Membrane Prep and 0.75 nM [125I]-(-)ICYP, a greater than 8-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 8-fold signal:background with 125I-labeled (-)Iodocyanopindolol at 0.75 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human beta 3 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_000025.1
Entrez基因汇总 The ADRB3 gene product, beta-3-adrenergic receptor, is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. 基因符号 - BETA3AR
- ADRB3
- ADRB3R
- B3AR
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 beta 3 靶子系列 Adrenergic UniProt编号 - P13945
UniProt汇总 FUNCTION: SwissProt: P13945 # Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
SIZE: 408 amino acids; 43519 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed mainly in adipose tissues.
SIMILARITY: SwissProt: P13945 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C
Radioligand: [125I]-(-) Iodocyanopindolol (Perkin Elmer # NEX189)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.产品使用声明 质量保证
SPECIFICATIONS: 1 unit = 10 µg membrane preparation10 µg/well Signal:Background 13.3 Specific Binding (cpm) 11074.7
Bmax: 1.3 pmol/mg
Kd: ~0.69 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,IPVH00010,Immobilon-P 卷膜，PVDF，0.45 µm，26.5 cm x 3.75 m
下一件merck millipore产品：merck millipore,默克密理博,04-221,Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal
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