merck millipore,默克密理博,04-221,Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal

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merck millipore,默克密理博,04-221,Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal
产品名称：Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal
产品型号：04-221
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin D1 Antibody, clone EP272Y, rabbit is an More>>

merck millipore,默克密理博,04-221,Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-221,Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	IHC, WB, ICC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-221
品牌系列	Upstate
商名	Upstate
描述	Anti-Cyclin D1 Antibody, clone EP272Y, rabbit monoclonal
Alternate Names	cyclin D1 (PRAD1: parathyroid adenomatosis 1) B-cell CLL/lymphoma 1 BCL-1 oncogene G1/S-specific cyclin D1 PRAD1 oncogene cyclin D1
背景信息	Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.

产品信息
格式	Culture Supernatant
控制	MCF-7 cell lysate.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin D1 Antibody, clone EP272Y, rabbit is an antibody against Cyclin D1 for use in IP, WB, IC, IH.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry: 1:50-100 dilution from a previous lot was used in immunocytochemistry. Immunoprecipitation: 1:50 dilution from a previous lot was used in immunoprecipitation.

生物信息
免疫原品种	KLH conjugated to Cyclin D1 linear peptide.
表位	N-terminus
克隆	EP272Y
宿主	Rabbit
特异性	This antibody recognizes Cyclin D1.
同种型	IgG
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_444284.1
Entrez基因汇总	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]
基因符号	U21B31 BCL1 D11S287E PRAD1
UniProt编号	P24385
UniProt汇总	Function: Essential for the control of the cell cycle at the G1/S (start) transition. Subunit structure: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Ref.10 Involvement in disease: A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus. Sequence similarities: Belongs to the cyclin family. Cyclin D subfamily

产品使用声明
质量保证	Routinely evaluated by western blot on MCF-7 cell lysate. Western Blot Analysis: A 1:1,000 dilution of this lot was used to detect Cyclin D1 on MCF-7 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.