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merck millipore,默克密理博,HTS144M,ChemiSCREEN™ Membrane Preparation Recombinant Human NK2 Tachykinin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human NK2 Tachykinin Receptor
产品型号：HTS144M
Human NK2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS144M,ChemiSCREEN™ Membrane Preparation Recombinant Human NK2 Tachykinin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS144M,ChemiSCREEN™ Membrane Preparation Recombinant Human NK2 Tachykinin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 NK2 描述 产品目录编号 HTS144M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human NK2 Tachykinin Receptor 概述 Full-length human TACR2 cDNA encoding NK2 背景信息 The tachykinin peptide family in mammals comprises three peptides, substance P, neurokinin A and neurokinin B, which bind to the tachykinin receptor family of GPCRs, NK1, NK2 and NK3 (Severini et al., 2002). Tachykinins have prominent activity in the GI system, in which they stimulate intestinal contraction and salivation; these effects are mediated by NK1 and NK2. Experiments with selective antagonists of NK2, SR 48,968 and MEN 10,376 indicate that NK2 mediates neurokinin- induced constriction of pulmonary artery and bronchus (Maggi et al., 1993). NK2 antagonists display efficacy in treatment of irritable bowel disorder (Lecci et al., 2004). Millipore's NK2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NK2 receptor interactions with its ligand. The membrane preparations exhibit a Kd of 0.76nM for [125I]-NKA. With 10.0 ug/well NK2 Membrane Prep and 0.5nM [125I]-NKA, a greater than 10-fold signal-to-background ratio was obtained. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.应用 应用 Human NK2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_001057.2
Entrez基因汇总 This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neuropeptide substance K, also referred to as neurokinin A. 基因符号 - TAC2R
- TACR2
- SKR
- NKNAR
- NK-2R
- NK2R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 NK2 靶子系列 Tackykinin /neurokinin UniProt编号 - P21452
UniProt汇总 FUNCTION: SwissProt: P21452 # This is a receptor for the tachykinin neuropeptide substance K (neurokinin A). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 398 amino acids; 44439 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P21452 ## Belongs to the G-protein coupled receptor 1 family.
MISCELLANEOUS: The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA, containing one Protease Inhibitor Cocktail Tablet (Roche Cat. No. 11 873 580 001) for each 50ml binding buffer.
Radioligand: [125I]-NKA (NEX 252)
Wash Buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled NKA at 0.5nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with 10ug/well of NK2 Receptor membrane prep.
SPECIFICATIONS: 1 unit = 10.0 µg membrane preparation10 µg/well Signal:Background 16.5 Specific Binding (cpm) 19906
Bmax: 2.21 pmol/mg
Kd: 0.76 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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下一件merck millipore产品：merck millipore,默克密理博,572660,SU11652 - CAS 326914-10-7 - Calbiochem
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