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merck millipore,默克密理博,HTS112M,ChemiScreen™ Recombinant Human Glucagon Receptor Membrane Preparation
产品名称：ChemiScreen™ Recombinant Human Glucagon Receptor Membrane Preparation
产品型号：HTS112M
Human glucagon / GCG GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS112M,ChemiScreen™ Recombinant Human Glucagon Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 glucagon / GCG 描述 产品目录编号 HTS112M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Recombinant Human Glucagon Receptor Membrane Preparation 概述 TRANSFECTION: Full-length human GCGR cDNA encoding Glucagon Receptor
HOST CELLS: Chem-1, an adherent mammalian cell line with no endogenous glucagon receptor expression.背景信息 Glucagon is a 29-amino acid peptide that stimulates glycogenolysis and gluconeogenesis in the liver to increase blood glucose. The receptor for glucagon is a class 2 (or class B) GPCR that signals through Gs to stimulate cAMP production (Mayo et al., 2003). Mice lacking the glucagon receptor have mild hypoglycemia after fasting, and exhibit hyperplasia of pancreatic α-cells (Gelling et al., 2003). Because of its role in promoting hyperglycemia, the glucagon receptor presents a potential target for treatment of diabetes. Chemicon's glucagon receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of glucagon receptor interactions and its ligands. The membrane preparations exhibit a Kd of 3.09 nM for [125I]-glucagon. With 1 nM [125I]-glucagon, 5 or 10 μg/well glucagon receptor Membrane Prep yields greater than 10-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA; no preservatives.
Packaging method: Membrane proteins were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human glucagon / GCG GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_000160.2
Entrez基因汇总 The physiologic effects of glucagon (GCG; MIM 138030) are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase.[supplied by OMIM] 基因符号 - GGR
- GCGR
- MGC138246
- GL-R
GPCR类别 B 宿主细胞 Chem-1 蛋白靶 glucagon / GCG 靶子系列 Glucagon UniProt编号 - P47871
UniProt汇总 FUNCTION: SwissProt: P47871 # This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
SIZE: 477 amino acids; 54009 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
DISEASE:SwissProt: P47871 # It is a candidate for certain defects in non-insulin- dependent diabetes mellitus (NIDDM). The variant in position 40 (Ser) is found in some NIDDM patients, but also in nondiabetic subjects.
SIMILARITY: SwissProt: P47871 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
• Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
• Radioligand: [125I]-glucagon (Perkin Elmer#:NEX-207)
• Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 10-fold signal:background with [125I]-labeled glucagon at 1 nM产品使用声明 质量保证 SPECIFICATIONS:
1 unit = 5 μg
Bmax for [125I] glucagon binding: 16.7 pmol/mg protein
Kd for [125I] glucagon binding: ~ 3.09 nM
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Glucagon receptor membrane prep:
10 µg/well 5 µg/well Signal:Background 10.1 35 Specific Binding (cpm) 33315 36252 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,16-251,Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,371543,Guanosine 5ʹ-O-(2-Thiodiphosphate), Trilithium Salt - CAS 97952-36-8 - Calbiochem
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