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merck millipore,默克密理博,HTS106M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT2 Melatonin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human MT2 Melatonin Receptor
产品型号：HTS106M
Human MT2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS106M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT2 Melatonin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS106M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT2 Melatonin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 MT2 描述 产品目录编号 HTS106M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human MT2 Melatonin Receptor 概述 Full-length human MTNR1B cDNA encoding MT2 背景信息 Melatonin (5-methoxy-acetyltryptamine) is a hormone synthesized and secreted in a circadian manner with high levels occurring in all species at night. Melatonin has been implicated in the regulation of various neural and endocrine processes that are cued by the daily change in photoperiod (Masana and Dubocovich, 2001) Two mammalian melatonin receptor subtypes, MT1 and MT2 have been cloned. Both receptors are members of the superfamily of putative seven transmembrane G-protein coupled receptors, which are coupled to the inhibition of cyclic AMP synthesis upon agonist binding. Pharmacological studies suggest that the phase shifting effect of melatonin on circadian rhythms may be mediated by the MT2 receptor subtype (Dubocovich et al. 1998). Melatonin inhibition of dopamine release from the retina is also mediated by the MT2 receptor subtype (Dubocovich et al. 1997). Chemicon's MT2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at MT2. The membrane preparations exhibit a Kd of 0.1 nM for [125I]-Iodomelatonin. With 0.2 nM [125I]-Iodomelatonin, 5 µg/well MT2 Membrane Prep typically yields greater than 6-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.应用 应用 Human MT2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_005959.3
Entrez基因汇总 This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin. 基因符号 - MEL-1B-R
- MTNR1B
- Mel-1B-R
- MT2
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 MT2 靶子系列 Melatonin UniProt编号 - P49286
UniProt汇总 FUNCTION: SwissProt: P49286 # High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
SIZE: 362 amino acids; 40188 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in retina and less in brain and hippocampus.
SIMILARITY: SwissProt: P49286 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 500mM NaCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C
Radioligand: [125I] Iodomelatonin (Perkin Elmer#: NEX-236)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 6-fold signal:background with [125I]-labeled Iodomelatonin at 0.2 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with MT2 membrane prep and unlabeled melatonin. 5 µg/well Signal:Background 9.96 Specific Binding (cpm) 1424.1
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [125I]-Iodomelatonin binding: 0.04 pmol/mg protein
Kd for [125I]-Iodomelatonin binding: ~0.1 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,341609,FGF Receptor Tyrosine Kinase Inhibitor IV, NP603 - CAS 949164-80-1 - Calbiochem
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