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merck millipore,默克密理博,06-1036,Anti-March5 Antibody
产品名称：Anti-March5 Antibody
产品型号：06-1036
Anti-March5 Antibody is a Rabbit Polyclonal Antibody for detection of March5 also known as Membrane-associated RING finger protein 5 & has been validated in WB, ICC.
merck millipore,默克密理博,06-1036,Anti-March5 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 06-1036 描述 Anti-March5 Antibody Alternate Names - Membrane-associated RING-CH protein V
- membrane-associated ring finger (C3HC4) 5
- Membrane-associated RING finger protein 5
- RING finger protein 153
- E3 ubiquitin-protein ligase MARCH5
- Mitochondrial ubiquitin ligase
背景信息 Membrane-associated RING-CH protein 5 (March5), also known as NKP66, is thought to be a mitochondrial ubiquitin ligase that is localized on the mitochondrial membrane. March5 has a Plant Homeo-Domain (PHD) motif that is essential for E3 ubiquitin ligase action. MARCH 5 is also thought to have four transmembrane domains. March5 has been associated with mitochondrial fission and division. March5 also seems to have a defensive role against mitochondrial dysfunction by interacting with mutant superoxide dismutase 1 (mSOD1) and preventing mSOD1 from causing damage. mSOD1 damage has been linked to the onset and progression of amyotrophic lateral sclerosis. 产品信息 格式 Serum 控制 - HL-60 cell lysate
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Anti-March5 Antibody is a Rabbit Polyclonal Antibody for detection of March5 also known as Membrane-associated RING finger protein 5 & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A representative lot of this antibody was used by an independent laboratory in IC. (Korfali, N., et al. (2010). Mol Cell Proteomics. 9:2571-2585.) 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human March5.
.宿主 Rabbit 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_060294
Entrez基因汇总 Summary: March5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM]. 基因符号 - RNF153
- MITOL
- MARCH-V
纯化方法 Unpurified UniProt编号 - Q9NX47
UniProt汇总 FUNCTION: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Authors show that the protein can be detected in endoplasmic reticulum. Authors show its presence only in mitochondria.
TISSUE SPECIFICITY: Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
DOMAIN: The RING-CH-type zinc finger domain is required for E3 ligase activity (by similarity).
POST-TRANSLATIONAL MODIFICATION: Autoubiquitinated leading to degradation (short half-life).
MISCELLANEOUS: By binding to and ubiquitinating two ALS1 variants of SOD1 (mSOD1 variants Arg-86 and Ala-94) it attenuates their cytotoxicity.
SEQUENCE SIMILARITIES: Contains 1 RING-CH-type zinc finger.产品使用声明 质量保证 Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected March5 on 10 µg of HL-60 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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