merck millipore,默克密理博,71059,RosettaBlue™(DE3) Competent Cells

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Merck Millipore 默克密理愽

merck millipore,默克密理博,71059,RosettaBlue™(DE3) Competent Cells - Novagen

描述
概述	RosettaBlue™ host strains are NovaBlue derivatives that combine high transformation efficiency and recA endA lacI^q mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
概述	Genotype: endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac (DE3) F′[proA⁺B⁺ lacI^qZΔM15::Tn10] pRARE (Cam^R, Tet^R)
产品目录编号	71059
品牌系列	Novagen®

产品信息
部件
保证高效	Guaranteed efficiency: > 1 x 10⁸ cfu/µg

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	≤ -70°C
无需冷冻	No
布鲁克黑文许可证	This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

merck millipore,默克密理博,71059,RosettaBlue™(DE3) Competent Cells - Novagen

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