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merck millipore,默克密理博,05-925R,Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal
产品名称：Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal
产品型号：05-925R
Detect MEK1 using this Anti-MEK1 Antibody, NT, clone C12T validated for use in WB, FC.
merck millipore,默克密理博,05-925R,Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC, WB Rb Purified Monoclonal Antibody 描述 产品目录编号 05-925R Replaces 04-376 描述 Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal Alternate Names - protein kinase, mitogen-activated, kinase 1 (MAP kinase<br />kinase 1)
- ERK activator kinase 1
- MAP kinase kinase 1
- MAPK/ERK kinase 1
- MAPKK 1
- mitogen-activated protein kinase kinase 1
背景信息 MAP Kinase/Erk Kinase (MEK), alternatively known as MKK, is a true dual-specificity kinase, in that it phosphorylates the MAP kinases on both the Thr and Tyr of the activation motif TEY. In vitro, the tyrosine phosphorylation is favored, whereas in vivo both phosphorylation events appear to occur simultaneously. This suggests that an additional factor is present in cells to facilitate the reaction. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser218/222 in MEK1 and Ser222/226 in MEK2), which are substrates for the Raf family of kinases. Mutation of the phosphorylation sites from Ser to Asp creates a protein with constitutive kinase activity, which when expressed in cells is able to cause transformation. 产品信息 格式 Purified 演示 Purified rabbit monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150 mM NaCl and 0.05% NaN3 with 40% glycerol. 应用 应用 Detect MEK1 using this Anti-MEK1 Antibody, NT, clone C12T validated for use in WB, FC. 主要应用 - Flow Cytometry
- Western Blotting
生物信息 免疫原品种 Recombinant peptide corresponding to amino acids surrounding the TEY motif in the activation loop of human phospho-MEK1. 表位 NT 克隆 C12T 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes MEK1 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_008927
Entrez基因汇总 The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. 基因符号 - MEK1
- EC 2.7.12.2
- PRKMK1
- MKK1
- MAPKK1
纯化方法 Protein A purfied UniProt编号 - Q02750
UniProt汇总 FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blotting on A431 cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody was used to detect MEK1 in A431 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,PIDL06P05,6-well Plate with Poly-D-Lysine Coating
下一件merck millipore产品：merck millipore,默克密理博,341609,FGF Receptor Tyrosine Kinase Inhibitor IV, NP603 - CAS 949164-80-1 - Calbiochem
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