merck millipore,默克密理博,HTS100RTA,Ready-to-Assay™ delta Opioid Receptor Frozen Cells

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merck millipore,默克密理博,HTS100RTA,Ready-to-Assay™ delta Opioid Receptor Frozen Cells
产品名称：Ready-to-Assay™ delta Opioid Receptor Frozen Cells
产品型号：HTS100RTA
Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS100RTA,Ready-to-Assay™ delta Opioid Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS100RTA,Ready-to-Assay™ delta Opioid Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	Delta / OP1 / DOP / DOR

描述
产品目录编号	HTS100RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ delta Opioid Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_000911
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	Delta / OP1 / DOP / DOR
靶子系列	Opioid
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.