merck millipore,默克密理博,ABS31,Anti-2A Peptide Antibody

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merck millipore,默克密理博,ABS31,Anti-2A Peptide Antibody
产品名称：Anti-2A Peptide Antibody
产品型号：ABS31
Anti-2A Peptide Antibody is an antibody against 2A Peptide for use in WB.

merck millipore,默克密理博,ABS31,Anti-2A Peptide Antibody

产品介绍

merck millipore,默克密理博,ABS31,Anti-2A Peptide Antibody

Replacement Information
Replacement Information	ABS31 is a direct replacement for 09-085

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
A	WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	ABS31
Replaces	09-085
描述	Anti-2A Peptide Antibody
背景信息	2A peptides and 2A-like peptide sequences (also known as CHYSEL, or cis-acting hydrolase elements) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, resulting in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. The use of 2A sequences allows the stoichiometric production of up to four proteins from a single vector, making it a powerful tool for the equimolar expression of multiple cistrons.

产品信息
格式	Serum
控制	2A-containing recombinant proteins
演示	Serum with 0.05% NaN3.

应用
应用	Anti-2A Peptide Antibody is an antibody against 2A Peptide for use in WB.
主要应用	Western Blotting

生物信息
免疫原品种	The immunogen used was recombinant 2A from foot and mouth virus1.
表位	The epitope is believed to be within the “GDVESNPG” region.
宿主	Rabbit
特异性	Recognizes the 2A sequence, derived from Foot and Mouth picornavirus (VKQTLNFDLLKLAGDVESNPGP), where represents the 2A cleavage site. The epitope is believed to be within the “GDVESNPG” region since the antibody also recognizes the unrelated 2A regions from Thosea asigna virus and Equine rhinitis A virus.
品种反应性	All
抗体类型	Polyclonal Antibody

产品使用声明
质量保证	Western Blot Analysis: A multicistronic vector was synthesized consisting of several CD3 proteins linked with 2A peptide sequences from foot and mouth disease, Thosea asigna, and Equine rhinitis A viruses, respectively (CD3δ-F2A-CD3ε-T2A-CD3γ-E2A-CD3ζ)1. The vector was transduced in 293 cells. A 1:1000 dilution of this antibody detected the 2A sequence in all three CD3-2A proteins (CD3 epsilon, gamma, delta) generated from a single 2A peptide-linked retroviral vector.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Western Blot Analysis:
A multicistronic vector was synthesized consisting of several CD3 proteins linked with 2A peptide sequences from foot and mouth disease, Thosea asigna, and Equine rhinitis A viruses, respectively (CD3δ-F2A-CD3ε-T2A-CD3γ-E2A-CD3ζ)1. The vector was transduced in 293 cells. A 1:1000 dilution of this antibody detected the 2A sequence in all three CD3-2A proteins (CD3 epsilon, gamma, delta) generated from a single 2A peptide-linked retroviral vector.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.