merck millipore,默克密理愽
  • merck millipore,默克密理博,HTS097RTA,Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells

  • merck millipore,默克密理博,HTS097RTA,Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells

    产品名称：Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells
    产品型号：HTS097RTA
    Human S1P3 / EDG3 GPCR Frozen Cells for calcium flux FLIPR Assays.

    merck millipore,默克密理博,HTS097RTA,Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells

  • 产品介绍
  • merck millipore,默克密理博,HTS097RTA,Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells

    重要规格表

    品种 宿主细胞 蛋白靶
    HumanChem-1S1P3 / EDG3
    描述
    产品目录编号 HTS097RTA
    商名
    • Ready-to-Assay
    描述 Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells
    背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

    Sphingosine 1-phosphate (S1P) is a bioactive lipid that binds to and activates a family of GPCRs, S1P1-5 (also known as EDG receptors). Interactions between S1P and its receptors mediate cytoskeletal rearrangement and cell migration, with functional consequences in angiogenesis, lymphocyte trafficking, and smooth muscle development (Anliker and Chun, 2004). S1P1 (Edg-1) signals exclusively through Gi, whereas S1P2 (Edg-5) and S1P3 (Edg-3) activate Gi, Gq and G12/13 (Windh et al., 1999). Although S1P1 and S1P3 promote cell migration, S1P2 inhibits cell migration in several cell types; these opposing functions appear to result from differences in the ability of each receptor to activate Gi (Sugimoto et al., 2003). Studies with knockout mice indicate that S1P2 and S1P3 have redundant functions in maintaining vascular integrity during embryonic development (Kono et al., 2004). In addition, S1P3 regulates immune responses by contributing to endothelial barrier function in splenic marginal zones (Girkontaite et al., 2004). Chemicon's cloned human S1P3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.
    产品信息
    部件
    • Fifty (50) mL of Media Component.
    • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
    应用
    应用 Human S1P3 / EDG3 GPCR Frozen Cells for calcium flux FLIPR Assays.
    生物信息
    品种 Human
    GPCR类别 A
    宿主细胞 Chem-1
    蛋白靶 S1P3 / EDG3
    靶子系列 Lysophospholipid
    细胞系类型
    • Ready-to-Assay
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
    储存和货运信息
    存储条件 Place cells in liquid nitrogen immediately upon receipt.
    包装信息
    数量 1 kit

    merck millipore,默克密理博,HTS097RTA,Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells

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