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merck millipore,默克密理博,HTS094M,ChemiSCREEN™ Membrane Preparation Recombinant Human GAL1 Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human GAL1 Receptor
产品型号：HTS094M
Human GAL1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS094M,ChemiSCREEN™ Membrane Preparation Recombinant Human GAL1 Receptor
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 GAL1 描述 产品目录编号 HTS094M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human GAL1 Receptor 概述 Human GAL1 背景信息 Galanin is a 29-30 amino acid peptide originally purified from intestine but later found to be abundant in the CNS. A family of 3 GPCRs, GAL1, GAL2 and GAL3, bind to galanin and mediate its biological effects. Galanin and its receptors have been implicated in pain, cognition, seizure activity, depression and drug addiction (Hokfelt, 2005). In particular, GAL1 mediates the anticonvulsant activity of galanin; mice lacking GAL1 are affected by spontaneous seizures, and have increased susceptibility to seizures induced by lithium-pilocarpine treatment and electrical perforant path stimulation (McColl et al., 2006; Mazarati et al., 2004). Chemicon's GAL1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GAL1 interactions with galanin. The membrane preparations exhibit a Kd of 1.7 nM for [125I]-galanin. With 5 ug/well GAL1 Membrane Prep and 0.25 nM [125I]-galanin, a greater than 50-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 50-fold signal:background with 125I-labeled galanin at 0.25 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human GAL1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Only Entrez基因编号 - NM_001480.2
Entrez基因汇总 The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart. 基因符号 - GALNR
- GALR1
- GALNR1
- GAL1-R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 GAL1 靶子系列 Galanin UniProt编号 - P47211
UniProt汇总 FUNCTION: SwissProt: P47211 # Receptor for the hormone galanin. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
SIZE: 349 amino acids; 38897 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
PTM: Palmitoylated on at least one of the three cysteine residues present in the C-terminal part.
SIMILARITY: SwissProt: P47211 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C Radioligand: [125I] galanin (Perkin Elmer # NEX333)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 SPECIFICATIONS: 1 unit = 5 ug membrane preparation Bmax: 19.2 pmol/mg Kd: 1.7 nM
10 µg/well 5 µg/well Signal:Background 77.6 75.6 Specific Binding (cpm) 38414 37384 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units 包装 1 mL per vial
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