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merck millipore,默克密理博,HTS092RTA,Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells
产品名称：Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells
产品型号：HTS092RTA
Human EP3 GPCR frozen cells for Calcium Flux FLIPR Assays.
merck millipore,默克密理博,HTS092RTA,Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells
- 产品介绍
重要规格表
品种 宿主细胞 蛋白靶 Human Chem-1 EP3 描述 产品目录编号 HTS092RTA 商名 - Ready-to-Assay
描述 Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells 背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGE2 causes pain, vasodilatation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. Further diversity is generated by alternative splicing; the human gene for EP3 generates 9 alternatively spliced mRNAs encoding 8 isoforms of EP3 (Kotani et al., 1997). These isoforms of EP3 vary in sequence at their C-termini, and differ in their ability to couple to Gs, Gq or Gi (Kotani et al., 1995). EP3 is required for fever induced by pyrogens, a response long attributed to prostaglandins by the antipyretic action of aspirin and other COX inhibitors (Ushikubi et al., 1998). In animal models of allergy, PGE2-mediated activation of EP3 inhibits inflammation to counteract the allergy-promoting activity of PGE2 (Kunikata et al., 2005). Millipore’s cloned human EP3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant EP3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP3 and its ligands.产品信息 部件 - Fifty (50) mL of Media Component.
- Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
应用 应用 Human EP3 GPCR frozen cells for Calcium Flux FLIPR Assays. 生物信息 品种 Human GPCR类别 A 宿主细胞 Chem-1 蛋白靶 EP3 靶子系列 Prostanoid 细胞系类型 - Ready-to-Assay
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
储存和货运信息 存储条件 Place cells in liquid nitrogen immediately upon receipt. 包装信息 数量 1 kit
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