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merck millipore,默克密理博,HTS071M,ChemiSCREEN™ Membrane Preparation Recombinant Human ChemR23 Chemoattractant Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human ChemR23 Chemoattractant Receptor
产品型号：HTS071M
Human ChemR23 / CMKLR1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS071M,ChemiSCREEN™ Membrane Preparation Recombinant Human ChemR23 Chemoattractant Receptor
- 产品介绍
- merck millipore,默克密理博,HTS071M,ChemiSCREEN™ Membrane Preparation Recombinant Human ChemR23 Chemoattractant Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 ChemR23 / CMKLR1 描述 产品目录编号 HTS071M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human ChemR23 Chemoattractant Receptor 概述 Full-length human ChemR23 背景信息 ChemR23 was discovered as an orphan receptor related to the chemoattractant receptors C3a, C5a and FPR1, and expressed on dendritic cells and macrophages (Samson et al., 1998). A ligand for ChemR23 was characterized as chemerin, a 15 kD proteolytically processed protein found in inflammatory sites; a 9 amino acid peptide from the C-terminus of chemerin is sufficient to activate ChemR23 (Wittamer et al., 2003, 2004). Chemerin expressed in lymphoid and microvascular endothelium mediates migration of ChemR23-expressing dendritic cells to lymphoid organs and vasculature at sites of inflammation (Vermi et al., 2005). In addition, a bioactive lipid, resolvin E1, was found to functionally interact with ChemR23 to reduce inflammation (Arita et al., 2005). Millipore's ChemR23 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of ChemR23 interactions with its ligands. The membrane preparations exhibit EC50s of 0.37 nM for chemerin in a GTPγS binding assay. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C应用 应用 Human ChemR23 / CMKLR1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_004072.1
基因符号 - CHEMR23
- CMKLR1
- ChemR23
- DEZ
- MGC126106
- MGC126105
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 ChemR23 / CMKLR1 靶子系列 Chemoattractant UniProt编号 - Q99788
UniProt汇总 FUNCTION: SwissProt: Q99788 # Orphan receptor. Could be a chemotactic peptide receptor. May have a function in bone metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
SIZE: 373 amino acids; 42322 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands.
DEVELOPMENTAL STAGE: Expressed during bone development.
SIMILARITY: SwissProt: Q99788 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled chemerin was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to a GF/B filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific chemerin-stimulated [35S]-GTPγS binding.
The ChemR23 membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product.产品使用声明 质量保证 SPECIFICATIONS: 1 unit = 5 μg
EC50 in GTPγS binding assay by Chemerin: ~ 0.37 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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