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品种反应性 主要应用 宿主 格式 抗体类型 M IHC, ELISA, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB2215 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Interferon-β Antibody 产品信息 格式 Serum 演示 Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl. 应用 应用 Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.
Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:
1. Put protein (approx 50ng) in blocking solution for 30-60 min RT
2. Add Primary antibody (1:500) to blocking solution
3. Agitate membrane for 60 min, RT
4. Wash 3X for 5min each with PBS-Tween
5. Add secondary antibody (1:1000) after diluting in PBS-Tween
6. Gentle agitation for 60min.
7. Wash 3X for 5min each with PBS-Tween
8. Develop with reaction kit of choice
Immunoprecipitation
Immunohistochemistry
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human IgG, whole molecule. 宿主 Rabbit 特异性 Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002176.2
基因符号 - IFNB
- IFNB1
- IFF
- MGC96956
- IFN-beta
- IFB
UniProt编号 - P01574
UniProt汇总 FUNCTION: SwissProt: P01574 # Has antiviral, antibacterial and anticancer activities.
SIZE: 187 amino acids; 22294 Da
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01574 ## Belongs to the alpha/beta interferon family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. 包装信息 数量 100 µL 包装 2x10(e4) units
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