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merck millipore,默克密理博,HTS064M,ChemiSCREEN™ Human Angiotensin II Receptor, Type I Membrane Preparation
产品名称：ChemiSCREEN™ Human Angiotensin II Receptor, Type I Membrane Preparation
产品型号：HTS064M
Human AT1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS064M,ChemiSCREEN™ Human Angiotensin II Receptor, Type I Membrane Preparation
- 产品介绍
- merck millipore,默克密理博,HTS064M,ChemiSCREEN™ Human Angiotensin II Receptor, Type I Membrane Preparation
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 AT1 描述 产品目录编号 HTS064M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human Angiotensin II Receptor, Type I Membrane Preparation 概述 Full-length human cDNA encoding AT1 背景信息 Angiotensin II (Ang II), an octapeptide produced by cleavage of angiotensinogen by angiotensin-converting enzyme, plays a fundamental role in cardiovascular homeostasis. Two GPCRs, AT1 and AT2, mediate the effects of AngII, although AT1 is primarily responsible for the effects of Ang II on renal function and development, thirst, electrolyte and water balance, and arterial blood pressure. Binding of Ang II to AT1 activates both Gq and Gi (De Gasparo et al., 2000). Chemicon's AT1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of AT1 interactions and its ligands. The membrane preparations exhibit a Kd of 0.36 nM for [125I]-Sar1-Ile8-Angiotensin II. With 0.3 nM [125I]-Sar1-Ile8-Angiotensin II, 5 µg/well AT1 Membrane Prep yields greater than 50-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80oC.应用 应用 Human AT1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_032049.2
- NM_000685.4
- NM_004835.3
- NM_009585.3
- NM_031850.2
Entrez基因汇总 Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least four transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. 基因符号 - AT1B
- AGTR1
- AT2R1A
- AT2R1B
- AGTR1B
- AT2R1
- AT1BR
- AGTR1A
- AT1AR
- AG2S
- HAT1R
- AT1
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 AT1 靶子系列 Angiotensin UniProt编号 - P30556
UniProt汇总 FUNCTION: SwissProt: P30556 # Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system.
SIZE: 359 amino acids; 41061 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Liver, lung, adrenal and adrenocortical adenomas.
PTM: C-terminal Ser or Thr residues may be phosphorylated.
DISEASE: SwissProt: P30556 # Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
SIMILARITY: SwissProt: P30556 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-Sar1-Ile8-Angiotensin II. (Perkin Elmer#:NEX-248 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 50-fold signal:background with 125I labeled Sar1-Ile8-Angiotensin II at 0.3 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of AT aub
1 membrane prep:
table
tr
th align="left"
/td
th align="center"
10 µg/well /td
th align="center"
5 µg/well /td
/tr
tr
td align="left"
Signal:Background /td
td align="center"
115.5 /td
td align="center"
92.8 /td
/tr
tr
td align="left"
Specific Binding (cpm) /td
td align="center"
46,458 /td
td align="center"
43,007 /td
/tr
/table
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [125I] Angiotensin II binding: 5.53 pmol/mg protein;
Kd for [125I] Angiotensin II binding: ~ 0.36 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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