merck millipore,默克密理博,HTS042M,ChemiScreen™ BLT1 Membrane Preparation

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merck millipore,默克密理博,HTS042M,ChemiScreen™ BLT1 Membrane Preparation
产品名称：ChemiScreen™ BLT1 Membrane Preparation
产品型号：HTS042M
Human BLT1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

merck millipore,默克密理博,HTS042M,ChemiScreen™ BLT1 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS042M,ChemiScreen™ BLT1 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPgammaS binding.	Human	Chem-1	BLT1

描述
产品目录编号	HTS042M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiScreen™ BLT1 Membrane Preparation
概述	Human BLT1
背景信息	BLT1 receptor is a Gi-coupled GPCR that binds primarily to the eicosanoid leukotriene B4. Macrophages, leukocytes and eosinophils express BLT1, and in vivo, BLT1 appears to mediate leukocyte migration and inflammation (reviewed in Brink et al., 2003). Chemicon's BLT1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of BLT1 interactions with Leukotriene B4. The membrane preparations exhibit a Kd of 1.3 nM for [3H]-Leukotriene B4. With 5.0 μg/well BLT1 Membrane Prep and 1 nM [3H]-Leukotriene B4, a signal-to-background ratio of approximately 3-fold is obtained.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human BLT1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
应用说明	Radioligand binding assay, and GTPgammaS binding.

生物信息
浓缩	1 mL, 0.5 mg/mL per vial.
品种	Human
Entrez基因编号	NM_181657.1
基因符号	LTB4R1 LTB4R BLTR BLT1 LTBR1 CMKRL1 P2Y7 GPR16 P2RY7 BLT
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	BLT1
靶子系列	Leukotriene
UniProt编号	Q15722
UniProt汇总	FUNCTION: SwissProt: Q15722 # Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. SIZE: 352 amino acids; 37557 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues. PTM: Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization. SIMILARITY: SwissProt: Q15722 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C Radioligand: [3H] Luekotriene (Perkin Elmer# NEX378) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 2.5-fold signal:background or 500 cpm with 3H-labeled Leukotriene B4 at 1 nM

产品使用声明

质量保证

SPECIFICATIONS:
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Motilin Receptor membrane prep:

	5 µg/well	10 µg/well
Signal:Background	75	65
Specific Binding (cpm)	46348	54457

1 unit = 5µg membrane preparation
Bmax: 5.6 pmol/mg
Kd: 0.43 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.