merck millipore,默克密理愽
  • merck millipore,默克密理博,HTS041M,ChemiScreen™ Bradykinin B2 Membrane Preparation

  • merck millipore,默克密理博,HTS041M,ChemiScreen™ Bradykinin B2 Membrane Preparation

    产品名称：ChemiScreen™ Bradykinin B2 Membrane Preparation
    产品型号：HTS041M
    Human B2 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

    merck millipore,默克密理博,HTS041M,ChemiScreen™ Bradykinin B2 Membrane Preparation

  • 产品介绍
  • merck millipore,默克密理博,HTS041M,ChemiScreen™ Bradykinin B2 Membrane Preparation

    重要规格表

    应用说明 品种 宿主细胞 蛋白靶
    Radioligand binding assay, and GTPgammaS bindingHumanChem-1B2
    描述
    产品目录编号 HTS041M
    品牌系列 Chemicon®
    商名
    • Chemicon
    • ChemiScreen
    描述 ChemiScreen™ Bradykinin B2 Membrane Preparation
    概述 Full length Human BDKRB2
    背景信息 Bradykinins bind and activate two GPCRs, termed B1 and B2 (gene names BDKRB1 and BDKRB2, respectively), which signal through Gq and Gi. B2 is widely expressed in a constitutive fashion, whereas B1 expression is induced during inflammation. B2 appears to mediate much of the activity of kinins in vasodilation, arthritis, edema, and nociception (Leeb-Lundberg et al., 2005). Chemicon's B2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of B2 interactions with bradykinin. The membrane preparations exhibit a Kd of 0.18 nM for [3H]-bradykinin. With 5.0 μg/well, B2 Membrane Prep and 0.4 nM [3H]-bradykinin, a greater than 4-fold signal-to-background ratio is obtained.
    产品信息
    演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.

    Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
    应用
    应用 Human B2 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
    应用说明 Radioligand binding assay, and GTPgammaS binding
    生物信息
    浓缩 1 mL, 0.5 mg/mL per vial
    品种 Human
    Entrez基因编号
    • NM_000623.2
    Entrez基因汇总 This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.
    基因符号
    • BK-2
    • BDKRB2
    • BK2
    • B2R
    • DKFZp686O088
    • BRB2
    • BKR2
    GPCR类别 A
    宿主细胞 Chem-1
    蛋白靶 B2
    靶子系列 Bradykinin
    UniProt编号
    • P30411
    UniProt汇总 FUNCTION: SwissProt: P30411 # Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    SIZE: 391 amino acids; 44461 Da
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Ubiquitous. Widespread in normal smooth muscle tissue and neurons.
    SIMILARITY: SwissProt: P30411 ## Belongs to the G-protein coupled receptor 1 family.
    孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

    Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C

    Radioligand: [3H] Bradykinin, [2,3-prolyl-3,4-3H(N)] (Perkin Elmer #NET-706)

    Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.

    One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 3H-labeled bradykinin at 0.4 nM
    产品使用声明
    质量保证 SPECIFICATIONS: 1 unit = 10 µg
    Bmax: 2.73 pmol/mg
    Kd: 0.18 nM
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
    包装信息
    数量 200 units

    merck millipore,默克密理博,HTS041M,ChemiScreen™ Bradykinin B2 Membrane Preparation

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下一件merck millipore产品：merck millipore,默克密理博,196805,Bax Channel Blocker - CAS 335165-69-0 - Calbiochem


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