merck millipore,默克密理博,04-402,Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal

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merck millipore,默克密理博,04-402,Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
产品名称：Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
产品型号：04-402
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibod More>>

merck millipore,默克密理博,04-402,Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-402,Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB, FC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-402
品牌系列	Upstate
商名	Upstate
描述	Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
Alternate Names	phosphatidylinositol 3 kinase gamma PIK3CG, PI3CG, PI3K, PI3Kgamma, PIK3, 1-phosphatidylinositol 3-kinase PI3-kinase PTDINS-3-kinase p110-gamma phosphatidylinositol 3 kinase gamma PIK3CG PI3CG PI3K PI3Kgamma
背景信息	PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

产品信息
格式	Purified
控制	Jurkat cell lysate
演示	Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110γ validated for use in FC, IP & WB.
主要应用	Western Blotting Flow Cytometry
应用说明	Immunoprecipitation: A 1:50 dilution of a previous lot of antibody immunoprecipitated PI3K, p110γ using protein G:agarose beads. Flow Cytometry: A 1:50 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the N-terminus human PI3 Kinase, p110γ
表位	N-terminus
宿主	Rabbit
特异性	This antibody recognizes the N-terminus human PI3 Kinase, p110γ.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Reactivity with other species has not been confirmed.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002649.2
Entrez基因汇总	This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
基因符号	p120-PI3K PIK3CG PI3CG p110-gamma PI3-kinase PI3Kgamma PTDINS-3-kinase PIK3 PI3K
纯化方法	Purified
UniProt编号	P48736
UniProt汇总	FUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. SIZE: 1102 amino acids; 126454 Da SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart. SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on jurkat cell lysate. Western Blot Analysis: A 1:1,000 dilution of the lot detected endogenous PI3 Kinase, p110γ in lysates from Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.