merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells

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merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells
产品名称：Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells
产品型号：HTS030RTA
Human PTH1 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	PTH1

描述
产品目录编号	HTS030RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Parathyroid hormone (PTH) plays a critical role in mineral ion homeostasis, particularly in bone and kidney (Mannstadt et al., 1999; Gensure et al., 2005). A related peptide, parathyroid hormone-related peptide (PTHrP), plays an important role in skeletal development. Both peptides exert their biological actions by binding to a class B GPCR, PTH1, that signals primarily by activation of adenylate cyclase (Schipani et al., 1993). Mutations in PTH1 have been identified as the cause of Jansen's metaphyseal chondrodysplasia, Blomstrand's chondrodysplasia, and enchondromatosis (Schipani and Provot, 2003). Intermittent treatment with PTH has important clinical utility in building bone mass in patients with osteoporosis (Rosen, 2003). Millipore’s cloned human PTH1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PTH1 and its ligands.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human PTH1 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_000316
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	PTH1
靶子系列	PTH receptor
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.