- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
描述 概述 The Perfectly Blunt® Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed (Novy 1996, Clark 1998, Brownstein 1996, Magnuson, 1996, Hu 1993). With the Perfectly Blunt cloning protocol, you can go from PCR product to plating transformants in less than one hour with minimal hands-on time. The finished PCR product is converted to a blunt, phosphorylated form in a 15-minute reaction using premixed reagents. Following a 5-minute heat inactivation step, the treated insert is combined with the ready-to-use vector and ligated in an optimized 15-min reaction. An exclusive 8-minute transformation procedure using highly efficient NovaBlue Singles™ Competent Cells (Cat. No. 70181) generates recombinant colonies that are easily visualized by blue/white screening.
Note that the Perfectly Blunt method is not limited to cloning PCR products; these kits are also suitable for cloning restriction fragments, cDNA, or sheared DNA with the same protocols.
Seven different vectors are available in Perfectly Blunt® Cloning Kits. Vector choices include those designed for general cloning, sequencing, optimal in vitro transcription/translation, and optimal protein expression in E. coli. Each vector is available in a kit containing sufficient reagents for 10, 20, or 40 reactions.
“Vector only” kits are also available in 20- and 40-reaction sizes without ligase and competent cells. For higher-efficiency competent cells, also consider the pSTBlue-1 Perfectly Blunt Giga Cloning Kit (Cat. No. 71229).
The pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.
In addition to the pETBlue™ Systems, which contain uncut plasmids, pETBlue vectors are available in formats ready for insertion of PCR products. Two types of kits are available:
AccepTor™ Vector Kits and Perfectly Blunt® Cloning Kits. The AccepTor Vector Kit enables direct insertion of DNA containing single 3′-dA overhangs, such as those amplified with non-proofreading DNA polymerases (e.g., NovaTaq™ DNA Polymerase), while the Perfectly Blunt Cloning Kits are designed for cloning inserts having any type of DNA end.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.产品目录编号 70636 品牌系列 Novagen®
特点及优点 - No restriction enzymes or special primers
- Compatible with any DNA polymerase
- Independent of 3ʹ-dA addition or sequence context of DNA ends
- Blue/white screening with seven different vectors, including pETBlue expression vectors
- Simple protocol takes less than one hour* from PCR product to plating transformants
* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
参考 参考 Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
产品信息 部件 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
无需冷冻 No
上一件merck millipore产品：merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells
下一件merck millipore产品：merck millipore,默克密理博,401607,Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate
merck millipore,默克密理博,553400,Radicicol, Diheterospora chlamydosporia - CAS 12772-57-5 - Calbiochem
merck millipore,默克密理博,AB3612,Anti-Caspase 12 Antibody, prodomain, aa 100-116
merck millipore,默克密理博,MABC136,Anti-ATG4A Antibody, clone EPR4122, rabbit monoclonal
merck millipore,默克密理博,HTS030RTA,Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells
merck millipore,默克密理博,401607,Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate
merck millipore,默克密理博,CBL1348,Anti-Integrin beta1 Antibody, clone HM beta 1.1
merck millipore,默克密理博,04-347,Anti-eIF-4E CT Antibody, rabbit monoclonal
merck millipore,默克密理博,AP181P,Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed
merck millipore,默克密理博,6510-OP,OmniPur Polyethylene Glycol 8,000 - CAS 25322-68-3 - Calbiochem