merck millipore,默克密理博,04-347,Anti-eIF-4E CT Antibody, rabbit monoclonal

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merck millipore,默克密理博,04-347,Anti-eIF-4E CT Antibody, rabbit monoclonal
产品名称：Anti-eIF-4E CT Antibody, rabbit monoclonal
产品型号：04-347
Detect eIF-4E CT using this Anti-eIF-4E CT Antibody, Rabbit validated for use in FC, IP, WB, IH(P).

merck millipore,默克密理博,04-347,Anti-eIF-4E CT Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-347,Anti-eIF-4E CT Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, FC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-347
品牌系列	Upstate
商名	Upstate
描述	Anti-eIF-4E CT Antibody, rabbit monoclonal
Alternate Names	D-eIF4E, Eif4e, IF4E, deIF4E, eIF-4E1, eIF-4E2, eIF-4EII, eIF-4e D-eIF4E Eif4e IF4E deIF4E eIF-4E1 eIF-4E2 eIF-4EII

产品信息
格式	Purified
演示	100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.

应用
应用	Detect eIF-4E CT using this Anti-eIF-4E CT Antibody, Rabbit validated for use in FC, IP, WB, IH(P).
主要应用	Western Blotting Flow Cytometry
应用说明	Suggested Dilutions: Immunoblot Analysis: 1:20,000 Immunoprecipitation: 1:140 Flow Cytometry: 1:40 Immunohistochemistry: 1:250-1:500

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to residues in the C-term of human eIF-4E.
宿主	Rabbit
特异性	Recognizes the C-terminal domain of eIF-4E.
同种型	IgG
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001968.2
Entrez基因汇总	All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
基因符号	EIF4F EIF4E eIF4E eIF-4E EIF-4E EIF4E1 CBP EIF4EL1 MGC111573
纯化方法	Serum
UniProt编号	P06730
UniProt汇总	FUNCTION: SwissProt: P06730 # Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. SIZE: 217 amino acids; 25097 Da SUBUNIT: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP- kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN (By similarity). Interacts with Lassa virus Z protein. PTM: Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. SIMILARITY: SwissProt: P06730 ## Belongs to the eukaryotic initiation factor 4E family.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

2 years at -20°C from date of shipment.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.