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merck millipore,默克密理博,HTS028M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst2 Somatostatin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human sst2 Somatostatin Receptor
产品型号：HTS028M
Human sst2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS028M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst2 Somatostatin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS028M,ChemiSCREEN™ Membrane Preparation Recombinant Human sst2 Somatostatin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 sst2 描述 产品目录编号 HTS028M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human sst2 Somatostatin Receptor 概述 Full-length human SSTR2 cDNA encoding sst2 背景信息 Somatostatin (sst) is a multifunctional peptide with two biologically active forms, sst-14 and sst-28, which are synthesized in neurons throughout the brain as well as in peripheral tissues such as the pancreas and the gut (Gillies, 1997). SST exerts a diverse array of effects that include inhibition of endocrine secretion, modulation of neurotransmission, and regulation of cell proliferation by stimulating a family of five G-protein-coupled receptors. Somatostatin receptor sst2 mRNA is predominantly expressed in central nervous system. Study using sst2 knock-out mice has found the increased anxiety-related behaviour while locomotor and exploratory activity was decreased in stress-inducing situations (coupled with an increase in pituitary ACTH release, a regulator of the stress response) (Viollet et al., 2000) . In the periphery, inhibition of glucagon release by sst in mouse islets is primarily mediated via sst2 (Strowski et al., 2000). In addition, endogenous sst functions through sst2 to suppress gastric acid secretion through inhibition of gastrin activity (Martinez et al., 1998). Millipore's sst2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of sst2. The membrane preparations exhibit a Kd of 0.95 nM for [125I]-Somatostatin 14. With 0.75 nM [125I]-Somatostatin 14, 5 μg/well sst2 Membrane Prep typically yields greater than 20-fold signal-to-background ratio. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C应用 应用 Human sst2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_001050.2
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 sst2 靶子系列 Somatostatin 孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-Somatostatin 14. (Perkin Elmer NEX-389 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 20-fold signal:background with 125I labeled somatostatin 14 at 0.75 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of sst2 Receptor membrane prep.
10 µg/well 5 µg/well Signal:Background 16.2 36.2 Specific Binding (cpm) 58438 54632
SPECIFICATIONS: 1 unit = 5 μg
Bmax for [125I]-Somatostatin binding: 18.9 pmol/mg protein
Kd for [125I]-Somatostatin binding: ~0.95 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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