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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB941 Replaces CBL410 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-S-100 Protein Antibody 产品信息 格式 Serum 演示 Rabbit serum. Liquid. Contains 0.1% sodium azide. 应用 应用 Anti-S-100 Protein Antibody detects level of S-100 Protein & has been published & validated for use in IH, IH(P). 主要应用 - Immunohistochemistry
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (6 microns thick) of human skin and squamous cell carcinomas were prepared. Cryostat sections of the above tissues may also be used.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated with phosphate buffered saline (PBS), pH 7.4.
2. The antiserum diluted 1:250-1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess reagents with PBS, the section is treated wit fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of the S-100 cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in the incubation time, e.g. 24 hours.生物信息 免疫原品种 Purified bovine S-100 bound to keyhole limpet hemocyanin (KLH) with carbodiimide. 宿主 Rabbit 特异性 The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of S-100-containing cells present in normal skin and human neoplasms using both the immunoperoxidase and immunofluorescence procedures. 品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006271.1
Entrez基因汇总 The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. 基因符号 - S100-alpha
- S100A1
- S100A
- S100
UniProt编号 - P04271
- P23297
UniProt汇总 FUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
SIZE: 94 amino acids; 10546 Da
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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