- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB5551 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neuregulin Antibody, type III Alternate Names - NRG1
背景信息 The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003). 产品信息 格式 Purified 控制 - Positive: Neonatal brain; Type III NRG1 is principally produced by
neurons. In peripheral nerve NRG1 would be present in and on axons.
演示 Purified immunoglobulin in liquid. 应用 应用 Anti-Neuregulin Antibody, type III is an antibody against Neuregulin for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 10-20 μg/mL;
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GST fusion protein from rat N terminal sequence (NRG1 III-beta1a) 表位 type III specific N-terminal cytoplasmic tail. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Neuregulin I, type III. Antibody recognizes both the proprotein (140kDa) and cleaved NTFm form (76kDa).
SPECIES REACTIVITY:
Rat. Reactivity with other species has not been determined. It is expected that the antibody may also react with human due to the homology of the immunogen. The antibody should equally recognize all Type III isoforms including beta2a, beta2b, and beta3.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001010848.2
基因符号 - pro-NRG3
- NRG3
- HRG3
- Pro-NRG3
UniProt编号 - P56975
UniProt汇总 FUNCTION: SwissProt: P56975 # Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
SIZE: 720 amino acids; 77901 Da
SUBCELLULAR LOCATION: Pro-neuregulin-3, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Does not seem to be active (By similarity). & Neuregulin-3: Secreted (By similarity). & Isoform 3: Cell membrane; Single-pass type I membrane protein. Note=Isoform 3 is also proteolytically released as a soluble form.
TISSUE SPECIFICITY: Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.DEVELOPMENTAL STAGE: Isoform 3 is expressed in fetal brain but not in other fetal tissues.
DOMAIN: SwissProt: P56975 The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization (By similarity). & ERBB receptor binding is elicited entirely by the EGF-like domain (By similarity).
PTM: Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. & Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
SIMILARITY: Belongs to the neuregulin family. & Contains 1 EGF-like domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. Avoid freeze thaws. Use sterile technique when handling. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,538927,15d-PGJ₂ - CAS 87893-55-8 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,105707,L-甲硫氨酸
相关产品
merck millipore,默克密理博,MSVMHTS0H,轴环支架，用于自动化
merck millipore,默克密理博,S7200,ApopTag Peroxidase In Situ Oligo Ligation (ISOL) Kit
merck millipore,默克密理博,PIRP15R48,Millicell 悬挂式细胞培养皿, PET, 1.0 µm
merck millipore,默克密理博,104233,双甘氨肽
merck millipore,默克密理博,538927,15d-PGJ₂ - CAS 87893-55-8 - Calbiochem
merck millipore,默克密理博,105707,L-甲硫氨酸
merck millipore,默克密理博,233501,Coagulation Factor XIII, Human Plasma
merck millipore,默克密理博,MAB353A4,Anti-Nestin Antibody, clone rat-401, Alexa Fluor 488 conjugate
merck millipore,默克密理博,16-236,Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 555