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merck millipore,默克密理博,HTS024M,ChemiSCREEN™ Membrane Preparation Recombinant Human CRF2 Neuropeptide Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human CRF2 Neuropeptide Receptor
产品型号：HTS024M
Human CRF2 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
merck millipore,默克密理博,HTS024M,ChemiSCREEN™ Membrane Preparation Recombinant Human CRF2 Neuropeptide Receptor
- 产品介绍
- merck millipore,默克密理博,HTS024M,ChemiSCREEN™ Membrane Preparation Recombinant Human CRF2 Neuropeptide Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPgammaS binding. Human Chem-1 CRF2 描述 产品目录编号 HTS024M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human CRF2 Neuropeptide Receptor 概述 Full-length human CRHR2 cDNA encoding CRF2 背景信息 The corticotropin-releasing factor receptors, CRF1 and CRF2, are Gs-coupled GPCRs expressed in the brain, blood vessels and intestine that bind to several neuropeptides, including corticotropin-releasing factor (CRF) and urocortin, and the amphibian peptide sauvagine (Lovenberg et al., 1995; Bale and Vale, 2004). In addition, two peptides, urocortin II (Ucn II) and urocortin III (Ucn III), bind selectively and with high affinity to CRF2 (Lewis et al., 2001). The CRF peptides and their receptors play important roles in stress mediated by the hypothalamic-pituitary-adrenal axis in animal models, and possibly in depression and anxiety in humans, although the contributions of CRF1 and CRF2 appear to be distinct (Bale and Vale, 2004; Risbrough et al., 2004) In addition, CRF1 and CRF2 differentially alter feeding behavior, gastric motility and vascular tone (Zorrilla et al., 2004; Martinez et al., 2004; Wiley and Davenport, 2004). Millipore's CRF2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CRF2 interactions with its ligands. The membrane preparations exhibit a Kd of 0.65 nM for [125I]-sauvagine. With 5 μg/well CRF2 Membrane Prep and 0.35 nM [125I]-sauvagine, a greater than 20-fold signal-to-background ratio is obtained. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human CRF2 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. 应用说明 Radioligand binding assay, and GTPgammaS binding. 生物信息 浓缩 0.5 mg/mL 品种 Human 品种反应性 Human Entrez基因编号 - NM_001883.3
基因符号 - CRF2R
- CRHR2
- CRH2R
- CRF2
- CRFR2
GPCR类别 B 宿主细胞 Chem-1 蛋白靶 CRF2 靶子系列 CRF Receptor UniProt编号 - Q13324
UniProt汇总 FUNCTION: SwissProt: Q13324 # This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 411 amino acids; 47688 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q13324 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] Sauvagine (Perkin Elmer#:NEX306)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 20-fold signal:background with 125I-labeled Sauvagine at 0.35 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with CRF2 membrane preparationn. 5 µg/well Signal:Background 64.6 Specific Binding (cpm) 38718
SPECIFICATIONS: 1 unit = 5 µg
Bmax: 9.04 pmol/mg protein
Kd : 0.65 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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