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merck millipore,默克密理博,HGPMAG-27K,MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
产品名称:MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
产品型号:HGPMAG-27K
The glycolysis pathway is a major energy source for the cell & is responsible for the conversion of glucose into pyruvate.
merck millipore,默克密理博,HGPMAG-27K,MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,HGPMAG-27K,MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 TKT, ENO1, G6PI, HIF-1α, LDHA, LDHB, PKM2 H Mplex Luminex xMAP 描述 产品目录编号 HGPMAG-27K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay 概述 The MILLIPLEX® MAP Human Glycolysis Pathway Magnetic Bead Panel (HGPMAG-27K). This panel is to be used for the simultaneous quantification of the following 7 analytes in any combination in cell or tissue lysates:
• ENO1: Enolase 1, α-enolase, C-myc promoter-binding protein, non-neural enolase
• G6PI: Glucose-6-phosphate isomerase, GPI
• HIF-1α: Hypoxia-inducible factor 1-α, ARNT-interacting protein
• LDHA: L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-M
• LDHB: L-lactate dehydrogenase B chain, LDH heart subunit, LDH-H
• PKM2: Pyruvate kinase isoform M2, Pyruvate kinase isozyme M2
• TKT: Transketolase, TK背景信息 The glycolysis pathway is a major energy source for the cell and is responsible for the conversion of glucose into pyruvate. The resulting pyruvate can then be converted into lactic acid in the cytoplasm or transferred to the mitochondria where it is converted into Acetyl-CoA which can enter the Krebs cycle and oxidative phosphorylation, generating ATP. Increased glucose uptake and a shift to aerobic glycolysis have been observed in many human tumors. This phenomenon is known as the Warburg effect. Glycolysis-related genes are highly upregulated genes in cancer. Monitoring and targeting metabolism in cancer cells is a rapidly expanding area of research, and deregulation of cellular energetics is now included as an emerging hallmark of cancer. 产品信息 检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度(%) - Intra-assay: <10%
- Inter-assay: <15%
板面类型 MAGNETIC Cellular Metabolism 应用 应用 The glycolysis pathway is a major energy source for the cell & is responsible for the conversion of glucose into pyruvate. 主要应用 - Multiplexing
应用说明 • Two hours incubation is recommended for best results.
• These assays require 25 µL diluted cell or tissue lysate.
• We recommend using 1-10 µg total protein/well.生物信息 特异性 Cross-reactivity between the antibodies and any of the other analytes in each panel is non-detectable or negligible. 品种反应性 Human 可用的分析物 - TKT
- ENO1
- G6PI
- HIF-1α
- LDHA
- LDHB
- PKM2
化验面板 Everything you need in a single kit. 交叉反应性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within this panel. 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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