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merck millipore,默克密理博,219440,PhosphoDetect™ Anti-Cdk1 (pTyr¹⁵) Rabbit pAb
产品名称：PhosphoDetect™ Anti-Cdk1 (pTyr¹⁵) Rabbit pAb
产品型号：219440
This PhosphoDetect™ Anti-Cdk1 (pTyr¹⁵) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Cdk1 (pTyr¹⁵).
merck millipore,默克密理博,219440,PhosphoDetect™ Anti-Cdk1 (pTyr¹⁵) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~34 kDa Cdk1 protein, phosphorylated at Tyr15, in hydroxyurea treated SaoS cell extracts. Also recognizes phosphorylated Cdk2. Does not react with Cdk4, Cdk6, Cdk7, or other phosphotyrosine proteins. 产品目录编号 219440 品牌系列 Calbiochem®
应用数据 
Detection of human Cdk1 phosphorylated at Tyr15 by immunoblotting. Samples: Whole cell lysate from Saos cells left untreated (left lane) or treated with hydroxyurea (middle lane) or nocodazole (right lane). Primary antibody: PhosphoDetect™ Anti-Cdk1 (pTyr15) Rabbit pAb (Cat. No. 219440) (1:1000). Detection: chemiluminescence.参考 参考 Norbury, C., and Nurse, P. 1992. Annu. Rev. Biochem. 61, 441.
Hunter, T. 1995. Cell 80, 225.
Galaktionov, K., et al. 1995. Genes Dev. 9, 1046.
Watanabe, K., et al. 1995. EMBO J. 14, 1878.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
McGwan, C.H. and Russel, P. 1993. EMBO J. 12, 75.产品信息 形式 Liquid 配方 In 150 mM NaCl, 10 mM sodium HEPES, 100 mg/ml BSA, 50% glycerol, pH 7.5. 正控制 Saos cells treated with hydroxyurea 防腐剂 None 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁵ phosphorylation site of human Cdk1 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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