merck millipore,默克密理博,04-230,Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal

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merck millipore,默克密理博,04-230,Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal
产品名称：Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal
产品型号：04-230
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-230,Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-230,Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC, IHC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-230
品牌系列	Upstate
商名	Upstate
描述	Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal
Alternate Names	histone deacetylase 3
背景信息	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone Deacetylase 3 (HDAC3) belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene that also plays critical roles in S phase progression and DNA damage control.

产品信息
格式	Culture Supernatant
控制	K562 whole cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunoprecipitation: 1:50 dilution from a previous lot was used for immunoprecipitation.

生物信息
免疫原品种	KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 3.
表位	C-terminus
克隆	Y415
宿主	Rabbit
特异性	This antibody recognizes Histone Deacetylase 3 at the C-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse and rat HDAC3.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003883
Entrez基因汇总	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq].
基因符号	SMAP45 EC 3.5.1.98 HD3 RPD3 RPD3-2
纯化方法	Unpurified
UniProt编号	O15379
UniProt汇总	FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Catalytic activity Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone. SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 By similarity. Interacts with CBFA2T3. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed. PTM: Sumoylated in vitro. Ref.14 SIMILARITY: Belongs to the histone deacetylase family. Type 1 subfamily.

产品使用声明
质量保证	Routinely evaluated by Western Blot on K562 whole cell lysate. Western Blot Analysis: A 1:10,000 dilution of this lot was used to detect Histone Deacetylase 3 in K562 whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.