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宿主 M 描述 概述 Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells. 产品目录编号 GR13L 品牌系列 Calbiochem®
同义词 Anti-EGF-R 参考 参考 Hunter T. 1984. Nature 311, 414.
Reynolds, F.H., et. al 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.产品信息 形式 Lyophilized 配方 Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA. 负控制 HL-60 cells 正控制 A431 cells 防腐剂 None 应用 应用参考 Original Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511. 主要应用 Immunoprecipitation
Neutralization Studies
生物信息 免疫原 partially purified, human EGFR from A431 cells 免疫原品种 Human 克隆 225 宿主 Mouse 同种型 IgG₁ 品种 human, not mouse, not rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 +2°C to +8°C
无需冷冻 No
特殊指南 Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
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