merck millipore,默克密理愽
  • merck millipore,默克密理博,FCCS025145,FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

  • merck millipore,默克密理博,FCCS025145,FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

    产品名称：FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit
    产品型号：FCCS025145
    This FlowCellect PLC-g1 Activation Dual Detection Kit for Flow Cytometry includes 2 conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor 488 & an Anti-PLC-g1-PE conjugate More>>

    merck millipore,默克密理博,FCCS025145,FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

  • 产品介绍
  • merck millipore,默克密理博,FCCS025145,FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

    描述
    产品目录编号 FCCS025145
    商名
    • FlowCellect
    描述 FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit
    概述 Millipore’s FlowCellect™ PLC-g1 Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor® 488 and an Anti-PLC-g1-PE conjugated antibody to measure total levels of PLC-g1. This two color flow cytometry kit is designed to detect the extent of PLC-g1 pathway activation by measuring PLC-g1 phosphorylation relative to the total PLC-g1 expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PLC-g1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-PLC-g1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.

    PLC-g1 is devoted to tyrosine kinase-dependent signaling for mitogenic responses to diverse agents such as growth factor activation of receptor tyrosine kinases and stimulation of immune cell receptors. Signaling pathways that depend on tyrosine kinases communicate with the PLC-g family. Because of its involvement in many key signaling cascades resulting from receptor tyrosine kinase activation, PLC-g1 plays a critical role in tumor progression and cancer-related illnesses. When stimulating A431 cells with EGF for 2 minutes the total protein level remains constant compared to unstimulated cells, while phospho-PLC-g1 levels are increased in all cells indicating that there is no competition between the two antibodies for their target epitopes.
    Alternate Names
    • Flow Cellect
    背景信息 Millipore’s FlowCellect™ PLC-gamma 1 Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-PLC-gamma 1 (Tyr837)-Alexa Fluor® 488 and an Anti-PLC-gamma 1-PE conjugated antibody to measure total levels of PLC-gamma 1. This two color flow cytometry kit is designed to detect the extent of PLC-gamma 1 pathway activation by measuring PLC-gamma 1 phosphorylation relative to the total PLC-gamma 1 expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PLC-gamma 1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-PLC-gamma 1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.

    PLC-gamma 1 is devoted to tyrosine kinase-dependent signaling for mitogenic responses to diverse agents such as growth factor activation of receptor tyrosine kinases and stimulation of immune cell receptors. Signaling pathways that depend on tyrosine kinases communicate with the PLC-gamma family. Because of its involvement in many key signaling cascades resulting from receptor tyrosine kinase activation, PLC-gamma 1 plays a critical role in tumor progression and cancer-related illnesses. When stimulating A431 cells with EGF for 2 minutes the total protein level remains constant compared to unstimulated cells, while phospho-PLC-gamma 1 levels are increased in all cells indicating that there is no competition between the two antibodies for their target epitopes.
    产品信息
    部件
    • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
    • 1. 20X Anti-phospho-PLC-g1 (Tyr783) Alexa Fluor® 488: (Part No. CS206377) One vial containing 150 µL.
    • 2. 20X Anti-PLC-g1-PE: (Part No. CS206378) One vial containing 150 µL.
    • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
    • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
    • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
    检测方法 Fluorescent
    应用
    应用 This FlowCellect PLC-g1 Activation Dual Detection Kit for Flow Cytometry includes 2 conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor 488 & an Anti-PLC-g1-PE conjugated antibody to measure total levels of PLC-g1.
    主要应用
    • Flow Cytometry
    生物信息
    宿主 Mouse
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    产品使用声明
    质量保证 Lot to lot in-process testing on all kit components
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
    储存和货运信息
    存储条件 4-8°C for antibodies and buffers
    包装信息
    数量 25 Tests / Kit

    merck millipore,默克密理博,FCCS025145,FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

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