merck millipore,默克密理博,GF053,Macrophage-Colony Stimulating Factor Protein, Recombinant human

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merck millipore,默克密理博,GF053,Macrophage-Colony Stimulating Factor Protein, Recombinant human
产品名称：Macrophage-Colony Stimulating Factor Protein, Recombinant human
产品型号：GF053
The M-CSF protein is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts & osteoblasts.

merck millipore,默克密理博,GF053,Macrophage-Colony Stimulating Factor Protein, Recombinant human

产品介绍

merck millipore,默克密理博,GF053,Macrophage-Colony Stimulating Factor Protein, Recombinant human

重要规格表

主要应用	品种	来源	纯度
CULT	Human	E. coli	Greater than 98% by SDS-PAGE and HPLC analyses

描述
产品目录编号	GF053
品牌系列	Chemicon®
商名	Chemicon
描述	Macrophage-Colony Stimulating Factor Protein, Recombinant human
概述	M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is involved in development, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Human M-CSF is a 36.8 kDa disulfide-linked homodimer composed of two 159 amino acid polypeptide subunits.
Alternate Names	M-CSF, CSF-1

产品信息
演示	The sterile filtered solution was lyophilized from sodium phosphate, pH 8.0 and 75 mM NaCl.

应用
应用	The M-CSF protein is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts & osteoblasts.
主要应用	Cell Culture
应用说明	For most in vitro applications, M-CSF exerts its biological activity in the concentration range of 0.1 to 20.0 ng/mL.

生物信息
纯度	Greater than 98% by SDS-PAGE and HPLC analyses
来源	E. coli
品种	Human
特异活性	The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is < 5 ng/mL, corresponding to a specific activity of > 2 x 10<sup>5</sup> units/mg.
Entrez基因编号	NM_172212.1 NM_000757.3 NM_172210.1 NM_172211.1
Entrez基因汇总	The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene.
基因符号	MGC31930 CSF1 M-CSF MCSF CSF-1 Lanimostim
UniProt编号	P09603
UniProt汇总	FUNCTION: SwissProt: P09603 # Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF- 1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. SIZE: 554 amino acids; 60119 Da SUBUNIT: Homodimer or heterodimer; disulfide-linked. SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein (By similarity). & Processed macrophage colony-stimulating factor 1: Secreted, extracellular space (By similarity). PTM: Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. & Isoform 1 is N- and O-glycosylated. Isoform 3 is N- glycosylated.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20° C. After a quick spin, reconstitute in water to a concentration of 0.1 - 0.5 mg/mL. Reconstituted M-CSF should be stored in undiluted aliquots at -20° C. Avoid repeated freeze/thaw cycles. This solution can then be further diluted into other buffers and stored at 2° - 8° C for one week or stored at -20° C for future use.

储存和货运信息

存储条件

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20° C. After a quick spin, reconstitute in water to a concentration of 0.1 - 0.5 mg/mL. Reconstituted M-CSF should be stored in undiluted aliquots at -20° C. Avoid repeated freeze/thaw cycles. This solution can then be further diluted into other buffers and stored at 2° - 8° C for one week or stored at -20° C for future use.