merck millipore,默克密理博,GF003-AF,Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant

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merck millipore,默克密理博,GF003-AF,Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant
产品名称：Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant
产品型号：GF003-AF
The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endotheli More>>

merck millipore,默克密理博,GF003-AF,Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant

产品介绍

merck millipore,默克密理博,GF003-AF,Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant

重要规格表

主要应用	品种	来源	纯度
CULT	Human	expressed in E. coli	Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of bFGF.

描述
产品目录编号	GF003-AF
品牌系列	Chemicon®
商名	Chemicon
描述	Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant
Alternate Names	Prostatropin FGF-2 bFGF basic-FGF Heparin-Binding Growth Factor 2
背景信息	Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.

产品信息
演示	Lyophilized from a 0.1 mg/mL solution of 5mM Tris, pH 7.6 with 150 mM NaCl.

应用
应用	The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
主要应用	Cell Culture
应用说明	For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/ml, depending on the method of culture

生物信息
纯度	Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of bFGF.
来源	expressed in E. coli
品种	Human
特异活性	Human FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.
Entrez基因编号	NM_002006.4
Entrez基因汇总	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
基因符号	FGFB FGF2 prostatropin Prostatropin HBGF-2 BFGF
UniProt编号	P09038
UniProt汇总	FUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SIZE: 155 amino acids; 17254 Da SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain the lyophilized material at -20°C until expiration date as stated on the label. General applications: After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity. For Human ES cell culture: After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).

储存和货运信息

存储条件

Maintain the lyophilized material at -20°C until expiration date as stated on the label.

General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).

包装信息
数量	50 µg

merck millipore,默克密理博,GF003-AF,Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant

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