- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,FCMAB113A4,Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate
产品名称：Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate
产品型号：FCMAB113A4
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
merck millipore,默克密理博,FCMAB113A4,Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, FC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 FCMAB113A4 描述 Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate Alternate Names - octamer-binding transcription factor-3
- Octamer-binding transcription factor 3
- POU class 5 homeobox 1
- POU domain class 5, transcription factor 1
- POU domain, class 5, transcription factor 1
- POU-type homeodomain-containing DNA-binding protein
背景信息 Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. 产品信息 格式 AlexaFluor®488 控制 - Human embryonic stem cells
演示 Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA. 应用 应用 Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC. 主要应用 - Immunocytochemistry
- Flow Cytometry
应用说明 Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Working Solution Cells / test Total Reaction Volume 5 μl 5 x 105 Cells / 95 μl PBS 100 μl 10 μl 1 x 106 Cells / 90 μl PBS 100 μl 生物信息 免疫原品种 Recombinant GST fusion protein, human Oct-4 (aa 1-134). 克隆 10H11.2 宿主 Mouse 特异性 Reacts with human Oct-4. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002701.4
基因符号 - MGC22487
- OCT3
- OCT4
- OTF3
- OTF4
- OTTHUMP00000029292
- Oct-3
- Oct-4
- Oct4
UniProt编号 - Q01860
UniProt汇总 FUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
INDUCTION: Transcriptional activity is positively regulated by PKM2.
DOMAIN: The POU-specific domain mediates interaction with PKM2.
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.产品使用声明 质量保证 Evaluated by Flow Cytometry in 2102Ep Cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt 包装信息 数量 100 tests
上一件merck millipore产品：merck millipore,默克密理博,208726,Calpain Inhibitor V - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,WB59,Western-Re-Probe™ Reagent
相关产品
merck millipore,默克密理博,516535,PERK Inhibitor I, GSK2606414 - CAS 1337531-89-1 - Calbiochem
merck millipore,默克密理博,07-1497,Anti-cAMP Antibody
merck millipore,默克密理博,17-656,ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
merck millipore,默克密理博,208726,Calpain Inhibitor V - Calbiochem
merck millipore,默克密理博,WB59,Western-Re-Probe™ Reagent
merck millipore,默克密理博,ABN174,Anti-MNX1 (HB9) Antibody
%5BAB1580-I_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,AB1580-I,Anti-Mu Opioid Receptor Antibody")
merck millipore,默克密理博,AB1580-I,Anti-Mu Opioid Receptor Antibody
merck millipore,默克密理博,70765,pET Expression System 22b - Novagen
merck millipore,默克密理博,MABN53,Anti-Dopamine D2 receptor (DRD2) Antibody, clone 3D9